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PfWRI1-10 transcription factor mediates lipid synthesis in Perilla frutescens through regulating PfBCCP1 gene
Xudong Chai1, Yali Zhou1, Jing Wen1
1College of Agriculture, Shanxi Engineering Research Center for Genetics and Metabolism of Specific Crops, Shanxi Agricultural University, Taigu, 030801, China.
Background:
Perilla frutescens is an oilseed crop rich in unsaturated fatty acids (UFAs). WRINKLED1 (WRI1) is an AP2/EREBP-type transcription factor (TF), which plays important regulation roles in fatty acid (FA) metabolism.
Results:
In this study, 11 PfWRI1 members were identified in the perilla genome. qRT-PCR analysis revealed that PfWRI1-10 was expressed higher than other members in developing seeds of perilla, suggesting that PfWRI1-10 may play crucial roles in seed oil biosynthesis. Overexpression of PfWRI1-10 resulted in enhancement of total oil content by 9.75% and α-linolenic acid (C18:3) level by 0.43%, while the soluble sugar content decreased in the transgenic tobacco leaves. qRT-PCR showed that the expression levels of glycolysis gene (NtPKp-β1) and FA biosynthesis genes (NtACP1 and NtBCCP1) significantly upregulated, whereas NtKAS1-B expression was downregulated in transgenic tobacco plants, indicating that PfWRI1-10 reallocated carbon sources flow to facilitate lipid biosynthesis by regulating the expression of the target genes. Yeast one-hybrid (Y1H) assays demonstrate that PfWRI1-10 directly binds to the AW-box cis-element of the PfBCCP1 promoter. Overexpression of PfBCCP1 enhanced the lipid content in the transgenic yeast, confirming its function in lipid metabolism.
Conclusions:
PfWRI1-10 transcription factor promotes lipid biosynthesis by upregulating PfBCCP1 and other lipid related genes. The current findings provide a scientific basis for the in-depth analysis of the regulatory network of perilla oil biosynthesis and also valuable genes for genetic improvement of perilla oil yield and quality.
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