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Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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CRISPR01:59

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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Updated: Apr 8, 2026

Using a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format
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Using a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format

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SDS-CRISPR for Single-Nucleotide Variant Detection.

Xin Guan1,2, Chong Guo1,2, Jiongyu Zhang1

  • 1Department of Biomedical Engineering, University of Connecticut Health Center, Farmington, Connecticut, USA.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
|April 7, 2026
PubMed
Summary
This summary is machine-generated.

Structure-Disruption-Sensitive CRISPR (SDS-CRISPR) enhances precision in detecting single-nucleotide variants, overcoming limitations of standard CRISPR-Cas12a systems for sensitive molecular diagnostics.

Keywords:
CRISPR‐based molecular diagnosticsIDH1 mutationSDS‐CRISPRglioma diagnosissingle‐nucleotide variant detection

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Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases
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Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases
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Area of Science:

  • Molecular Biology
  • Genomics
  • Biotechnology

Background:

  • CRISPR-Cas12a is a powerful tool for nucleic acid diagnostics but struggles with single-nucleotide variant (SNV) detection due to mismatch tolerance.
  • Accurate SNV detection is crucial for applications like cancer variant identification and infectious disease diagnostics.

Purpose of the Study:

  • To develop a highly precise CRISPR-Cas12a-based system for allele discrimination and SNV detection.
  • To elucidate the structural mechanisms underlying SNV discrimination in the novel system.
  • To enable rapid, on-site molecular diagnostics for clinical applications.

Main Methods:

  • Development of Structure-Disruption-Sensitive CRISPR (SDS-CRISPR) utilizing AlphaFold3 modeling and bioinformatic analysis.
  • Structural and ionic modulation of Cas12a to enhance SNV discrimination.
  • Application of SDS-CRISPR for detecting IDH1 alleles in glioma samples and HIV-1 drug-resistance mutations.
  • Integration with lateral-flow strips and AI-assisted smartphone readers for point-of-care testing.

Main Results:

  • SDS-CRISPR achieved attomole sensitivity and detected variants at 0.01% frequency for IDH1 alleles.
  • On-site detection was achieved within 20 minutes using the integrated system.
  • Clinical validation with glioma tissues showed high concordance with immunohistochemistry.
  • Plasma cfDNA analysis demonstrated mutation fractions consistent with next-generation sequencing.
  • SDS-CRISPR successfully discriminated microRNA isoforms and identified HIV-1 drug-resistance mutations.

Conclusions:

  • SDS-CRISPR provides a mechanistically informed and highly precise method for allele discrimination.
  • The system offers a universal framework for sensitive molecular diagnostics, applicable to various targets including SNVs, microRNAs, and infectious agents.
  • SDS-CRISPR holds significant potential for clinical applications, including intraoperative diagnostics and precision medicine.