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Method for the Isolation of Francisella tularensis Outer Membranes
Published on: June 29, 2010
Physicochemical and Antigenic Properties of Experimental Preparations of Francisella tularensis
A V Korneva1, V B Nikolaev2, E Y Markov2
1Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Irkutsk Antiplague Research Institute, Irkutsk, Russia. korneva@inbox.ru.
We analyzed physicochemical properties and antigenic activity of cell envelope preparations isolated from urea lysates of different Francisella tularensis subspecies. Lysis of F. tularensis cells with a urea solution yielded polyepitopic immunogenic complexes with high antigenic activity detected in specific immunochemical reactions (dot immunoassay, immunoblotting, radial immunodiffusion) with IgG isolated from commercial horse tularemia serum and labeled with colloidal silver nanoparticles.
We analyzed physicochemical properties and antigenic activity of cell envelope preparations isolated from urea lysates of different Francisella tularensis subspecies. Lysis of F. tularensis cells with a urea solution yielded polyepitopic immunogenic complexes with high antigenic activity detected in specific immunochemical reactions (dot immunoassay, immunoblotting, radial immunodiffusion) with IgG isolated from commercial horse tularemia serum and labeled with colloidal silver nanoparticles.
