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Optimal 1TEL-target protein linker character is target protein-dependent

Maria J Pedroza Romo1, Alihikaua Keliiliki1, Jacob C Averett1

  • 1Department of Chemistry and Biochemistry, Brigham Young University, 701 East University Parkway, Provo, UT 84602, USA.

Acta Crystallographica. Section D, Structural Biology
|April 7, 2026
PubMed
Summary

Selecting the right linker is key for protein crystallization using the 1TEL chaperone. Short, rigid, or semi-flexible linkers work best for some proteins, while flexible linkers are optimal for others, improving crystal quality.

Keywords:
1TELDARPinsETV6TELSAMdesigned ankyrin-repeat proteinslinkersprotein binding

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