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Updated: Apr 9, 2026

Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes
Published on: November 23, 2021
Small molecule fluorescent probes based carbazole-pyridinium salts: one- and two-photon excited fluorescence and
Abstract:
Two pyridinium salts fluorescent molecules based on carbazole (BDY and BDD) were designed and synthesized, and their photophysical properties and application in bioimaging were systematically investigated. It was found that both compounds exhibited large Stokes shifts and high fluorescence quantum yields. Furthermore, remarkable two-photon excited fluorescence was demonstrated under excitation by a 720-1020 nm near-infrared femtosecond laser. Both compounds were successfully applied in cell staining and bioimaging, where excellent fluorescence signals were demonstrated. In summary, owing to their outstanding optical properties, these pyridine salt derivatives are deemed to possess great potential for application as high-performance fluorescent probes in the field of biomedical imaging.
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