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Updated: Apr 9, 2026

Optimized Quantitative Assessment of Enhancer RNA Stability in Mouse Embryonic Stem Cells
Published on: November 21, 2025
Single-oocyte full-length isoform sequencing unveils the impact of transposable elements on RNA diversity and
Yuqian Wang1,2,3,4, Wei Wang1,2,3,4,5,6, Yujun Liu1,2,3,4
1State Key Laboratory of Female Fertility Promotion, Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, China.
Abstract:
Oocyte-specific isoforms play crucial roles in oocyte maturation, while current understanding of the oocyte transcriptome is mainly focused on gene level. Here, we utilize single-cell full-length isoform sequencing to detect entire transcripts in human and mouse oocytes. Isoform diversity during oocyte maturation is systematically profiled, including 7154 and 4875 putative novel human and mouse transcripts, respectively. More than half of novel isoforms are categorized as novel-not-in-catalog (NNC) and may serve specific functions in oocytes. For example, ARHGAP18 mainly encoded by novel isoforms colocalizes with microtubules, and targeted knockdown of novel isoforms disrupts oocyte maturation. Moreover, approximately 30% of NNC isoforms are derived from transposable elements, and their incorporation within transcripts could enhance isoform stability during oocyte maturation. Altogether, our findings represent a valuable resource showcasing the complexity and diversity of RNA isoforms in oocytes, as well as transposable element co-option for novel isoform generation and isoform stability enhancement.
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