Related Experiment Video

Updated: Apr 9, 2026

Sequencing Small Non-coding RNA from Formalin-fixed Tissues and Serum-derived Exosomes from Castration-resistant Prostate Cancer Patients
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Sequencing Small Non-coding RNA from Formalin-fixed Tissues and Serum-derived Exosomes from Castration-resistant Prostate Cancer Patients

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NSUN2/ALYREF-mediated RNA m5c modification promotes anoikis resistance of prostate cancer through activating

Guangyu Sun1, Shuaiqi Chen2, Mingzhe Chen1

  • 1Department of Urology, Tianjin Medical University General Hospital, Tianjin, China.

Oncogene
|April 7, 2026
PubMed

Insights

This study reveals that ALYREF protein promotes prostate cancer progression by enhancing anoikis resistance and metastasis through m5C modification of BNIP3 mRNA, identifying it as a potential therapeutic target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Epigenetics

Background:

  • Prostate cancer is a leading global health concern for aging men.
  • RNA 5-methylcytosine (m5C) modification is implicated in various cancers.
  • The function of ALYREF in prostate cancer remains largely unknown.

Purpose of the Study:

  • To investigate the role of ALYREF in prostate cancer.
  • To elucidate the mechanism by which ALYREF influences cancer progression.
  • To identify ALYREF as a potential therapeutic target for advanced prostate cancer.

Main Methods:

  • Knockdown of ALYREF in prostate cancer cells.
  • Analysis of proliferation, invasion, and metastasis.
  • Investigation of anoikis resistance and autophagy.
  • Identification of ALYREF binding to BNIP3 mRNA m5C site.

Main Results:

  • Knockdown of ALYREF significantly reduced prostate cancer proliferation, invasion, and metastasis.
  • ALYREF enhances anoikis resistance by upregulating BNIP3 and activating autophagy.
  • ALYREF directly binds to the m5C site of BNIP3 mRNA, increasing its stability.
  • This mechanism enhances prostate cancer cell survival and metastatic potential.

Conclusions:

  • ALYREF plays a crucial role in prostate cancer progression.
  • ALYREF-mediated m5C modification of BNIP3 mRNA is key to autophagy and anoikis resistance.
  • ALYREF represents a promising molecular target for advanced prostate cancer treatment.

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