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Updated: Apr 9, 2026

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Sizing single trapped nanoparticles with interferometric scattering fluctuations.

Abhijit A Lavania1, William B Carpenter2,3

  • 1Department of Applied Physics, Stanford University, Stanford, CA, USA.

Physical Chemistry Chemical Physics : PCCP
|April 8, 2026
PubMed
Summary
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Researchers can now measure single nanoparticle sizes using label-free interferometric scattering microscopy. This technique analyzes contrast fluctuations within an interferometric scattering anti-Brownian electrokinetic (ISABEL) trap to determine hydrodynamic diameter.

Area of Science:

  • Nanotechnology
  • Biophysics
  • Microscopy

Background:

  • Nanoparticle size critically impacts properties, necessitating precise single-particle quantification.
  • Interferometric scattering microscopy offers sensitive, label-free detection with axial information.
  • Existing methods struggle with accurate single-nanoparticle size determination.

Purpose of the Study:

  • To develop a label-free method for hydrodynamic sizing of single nanoparticles.
  • To utilize interferometric scattering contrast fluctuations for size estimation.
  • To validate the technique on known standards and biological nanoparticles.

Main Methods:

  • Employing an interferometric scattering anti-Brownian electrokinetic (ISABEL) trap for nanoparticle confinement.

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Last Updated: Apr 9, 2026

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  • Analyzing fluctuations in interferometric scattering contrast using time autocorrelation.
  • Interpreting autocorrelation data with a Brownian Dynamics model to estimate hydrodynamic diameter.

    • Main Results:

    • Successfully demonstrated hydrodynamic sizing of single gold and polystyrene nanoparticles.
    • Verified the correlation between scattering contrast fluctuations and particle size.
    • Applied the method to determine the size of single carboxysomes from bacteria.

    Conclusions:

    • Interferometric scattering contrast fluctuations in an ISABEL trap enable label-free hydrodynamic sizing of single nanoparticles.
    • The developed method provides a sensitive platform for advanced nanoparticle analysis.
    • This technique enhances capabilities for studying nanoscale objects without labeling.