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First 20 Years of Orbitrap Mass Spectrometry as the Mainstream Analytical Technique
1Thermo Fisher Scientific (Bremen) GmbH, Bremen, Germany.
Mass Spectrometry Reviews
|April 8, 2026
Summary
Orbitrap mass spectrometry has revolutionized high-resolution and accurate-mass (HR/AM) analysis over 20 years. Innovations in Orbitrap technology have made HR/AM accessible and expanded its applications in diverse scientific fields.
Area of Science:
- Analytical Chemistry
- Spectrometry
- Instrument Development
Background:
- Orbitrap mass spectrometry emerged as a significant high-resolution and accurate-mass (HR/AM) technology.
- Its development over two decades has seen substantial advancements in instrumentation and applications.
Purpose of the Study:
- To review the historical development and technological innovations of Orbitrap mass spectrometry.
- To highlight its widespread adoption and diverse applications in scientific research.
Main Methods:
- Historical review of Orbitrap analyzer development.
- Analysis of major instrument families and technological advancements.
- Examination of hybrid and Tribrid architectures and benchtop platforms.
Main Results:
- Orbitrap technology has been democratized, becoming accessible through benchtop platforms.
- Significant extensions in high-mass analysis, native analysis, and integration with ion mobility.
- Successful application in structural biology, isotope ratio measurements, and space research.
Conclusions:
- Orbitrap instrumentation has become a workhorse in analytical laboratories globally.
- Future directions indicate continued evolution and expanded roles in scientific analysis.
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