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Updated: Apr 9, 2026
Calvarial Model of Bone Augmentation in Rabbit for Assessment of Bone Growth and Neovascularization in Bone Substitution Materials
Published on: August 13, 2019
Histomorphologic Assessment of Osteoregeneration in a Rabbit Femur Model With Xenograft, Bone Morphogenetic
Berik Tuleubayev1, Yevgeniy Kamyshanskiy2, Yerkin-Dauir Kurmangaliyev3
1From the Department of Surgical Diseases, Karaganda Medical University, Karaganda, Kazakhstan.
Background:
This study investigated whether the combination of thermally processed bone xenograft (XG) and recombinant human bone morphogenetic protein-2 (rhBMP-2) with platelet-rich plasma (PRP) or Prolia (denosumab) can enhance bone regeneration in a rabbit femoral bone defect model.
Methods:
Seventy-two rabbits with created femoral bone defects (5 mm) were divided into 4 groups: (1) XG + rhBMP-2 + Prolia; (2) XG + rhBMP-2 + PRP; (3) XG + rhBMP-2; and (4) XG. At 14, 30, and 60 days after implantation, samples were prepared for histological and histomorphometric analysis.
Results:
Histomorphometrically, on day 30, there were no statistically significant differences between groups regarding the amount of newly formed bone (P = 0.2231), but a reduction in residual graft in the intramedullary space was observed in the PRP group. On day 60, more intense formation of new bone with reduced intragroup variation in the amount of newly formed bone was found in the XG + rhBMP-2 + PRP group (P < 0.001). At day 60, XG + rhBMP-2 + Prolia showed no cortical bone increase because of concurrent resorption.
Conclusions:
Combined therapy using thermally processed bone graft, rhBMP-2, and PRP improves bone healing in femoral defects in rabbits.
