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Dual-Wavelength PDT With Chlorin-Based Photosensitizers for Oral Wounds Healing: Study of Proliferative Activity and
M A Shakhova1,2, E A Durnovo1, N Yu Orlinskaya1
1Privolzhsky Research Medical University, Nizhny Novgorod, Russia.
Background:
The aim of the study is the comparative analysis of the effectiveness of photodynamic therapy in the management of the wound surface of the oral mucosa of rats using combined dual-wavelength photodynamic therapy (PDT).
Methods:
The study was performed on 36 females outbred Wistar stock rats. The animals were divided into control (n = 18) and PDT (n = 18) groups. Wound surfaces were modeled on the cheek mucosa. Dual-wave photodynamic therapy was performed on the 1st postoperative day in the PDT group. An immunohistochemical study with Ki67 and CD31 markers was performed on days 3, 7, and 14 to determine the proliferative activity and assess the state of the microvascular bed.
Results:
Macroscopic examination of the tissues of the wound surface after a single photodynamic exposure revealed the acceleration of healing processes and the reduction in the time of tissue reorganization compared to the control group. The stratum corneum of the epithelium, which serves as one of the main protective mechanisms, was completely restored on day 7. The peak of proliferative activity occurred on day 3, which indicated the earlier activation of regenerative processes after photodynamic exposure. The highest expression rates of the CD31 endothelial marker in the early phase of the wound process in the PDT group indicate the active formation of the microvascular bed.
Conclusion:
The use of photodynamic therapy is an effective way to activate and intensify regenerative processes in tissues due to timely restoration of trophism and provision of oxygen to tissues.
