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Updated: Apr 9, 2026

Applications of pHluorin for Quantitative, Kinetic and High-throughput Analysis of Endocytosis in Budding Yeast
Published on: October 23, 2016
Polarized anionic phospholipids and exocytosis are implicated in the polarized recruitment of budding yeast AP180, an
Paul Marchando1, Gean Hu1, Feng Yuan1
116 Barker Hall, Deptartment of Molecular and Cell Biology, University of California, Berkeley, CA 94720.
Abstract:
Understanding of the mechanisms that initiate clathrin-mediated endocytosis (CME) is incomplete. Recent studies in budding yeast identified the endocytic adaptor proteins Yap1801/Yap1802 (budding yeast AP180) as key CME factors that promote CME initiation in daughter cells during polarized growth, but how Yap1801/2 are recruited preferentially to the plasma membrane of daughter cells is not clear. The only known cargos for Yap1801/2 in yeast are the synaptobrevins Snc1 and Snc2, which serve as v-SNARES for exocytic vesicles reaching the plasma membrane and are crucial for polarized cell growth. In this study, we examine the spatiotemporal dynamics of functional, fluorescent protein-tagged Snc2 expressed from its endogenous locus and provide evidence that, along with anionic phospholipids, Snc2 specifically recruits Yap1802 to growing daughter cells. This protein-protein interaction creates a direct link between polarized secretion and CME and has further implications in CME initiation.
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