Regulation of wound pathogens by electrode generated impulses targeting log phase and quorum sensing pathways

Electronics in the field of health care endures rapid changes. It covers a wide range of therapeutic pathways and medical devices. Wound therapy mediated by electrical impulse has the potential to reduce bacterial growth and enhance the healing of wounds. In this work, we were targeting bacterial cell membrane by means of electrode generated impulse for log phase restriction and quorum sensing-dysregulation. A low voltage electrical impulse significantly reduced the bacterial growth up to 80.8% for E.coli, 81.38% for P.aeruginosa, 67.2% for S.aureus and 36.55% for E. faecalis. The impluse affects the selective permeability of the bacterial cell membrane by causing electrochemical changes at the membrane, restricting the bacterial multiplication. Transient pore formation in bacterial membrane was confirmed by the release of lactate dehydrogenase by both gram-negative (65%) and gram-positive bacteria (16.7%). Quorum sensing dysregulation occurred due to the disruption of exopolysaccharides up to 45% for S.aureus and biofilm up to 35% for P.aeruginosa. This influences the bacterial multiplication as reduction in exopolysaccharides hinders the communication between bacteria. The bacterial respiratory chain varies by 16-80. This results in the disruption of energy production and metabolic imbalance in bacteria which ultimately supresses the bacterial proliferation.