Related Experiment Video
Updated: Apr 10, 2026
09:27
Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability
Published on: April 22, 2016
18.4K
Collaborative self-assembled enzymes and self-immobilised biofilms for enhanced biocatalytic processes
Peifang Ren1, Wenjun Sun1, Chaowei Zhou1
1National Engineering Research Center for Biotechnology, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, China; State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, China.
Bioresource Technology
|April 8, 2026
Summary
This study introduces a novel biocatalytic platform for continuous bio-based product manufacturing. The method uses co-immobilization to maintain high enzyme efficiency over multiple batches, overcoming key challenges in biocatalysis.
Area of Science:
- Biocatalysis
- Biotechnology
- Enzyme Engineering
Background:
- Continuous production of bio-based products is a major hurdle in biocatalysis.
- Existing methods face challenges in maintaining enzyme efficiency over extended periods.
Purpose of the Study:
- To develop a novel one-step biocatalytic platform for continuous production.
- To address the challenge of enzyme deactivation and loss in multi-batch processes.
Main Methods:
- Co-immobilization of enzymes on cell surfaces and carrier-bound biofilms.
- Utilizing surface-displayed enzymes for continuous catalytic conversion.
- Testing the platform with pullulanase for substrate hydrolysis and resistant starch production.
Main Results:
- Achieved over 99% conversion rate for pullulan in 16 batches with pullulanase.
- Demonstrated a space-time hydrolysis rate of 20 fg/L/d.
- Successfully produced resistant starch type 3 from natural starch.
Conclusions:
- The novel co-immobilization platform enables efficient, continuous biocatalysis.
- This strategy offers a promising solution for overcoming multi-batch catalysis challenges.
- The platform is highly suitable for controlled environment biological catalysis.
Related Concept Videos
01:29Biofilms
2.0K
Biofilms are complex communities of microorganisms encased in a self-produced extracellular polysaccharide matrix attached to surfaces. These microbial consortia can include single or multiple species, providing enhanced survival benefits by forming organized, multilayered structures.The formation of biofilms occurs through four key stages: attachment, colonization, development, and dispersal.During attachment, free-swimming planktonic cells adhere to a surface, often facilitated by...
2.0K
01:29Bioreactor Design and Operational System
124
Bioreactors are engineered vessels designed to cultivate microorganisms under controlled conditions for industrial bioprocessing. They maintain sterility and allow precise regulation of pH, temperature, oxygen, and nutrient levels to optimize microbial growth and metabolite production. Bioreactors range from small laboratory units of 1 liter to industrial systems holding up to 500,000 liters, though only about 75% of their volume is actively used for fermentation. The remaining headspace...
124
01:25Biofuels
84
The microbial conversion of organic matter into biofuels holds potential as a renewable energy source. Among biofuel sources, microalgae are recognized as a highly efficient and adaptable feedstock for biodiesel production, owing to their rapid biomass accumulation, elevated lipid productivity, and capacity to proliferate in diverse aquatic systems, including freshwater, marine, and wastewater habitats. Unlike terrestrial crops, microalgae do not compete for land and can achieve significantly...
84
01:22Bioreactor Controls-III
54
Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...
54
02:34Enzymes
97.4K
Inside living organisms, enzymes act as catalysts for many biochemical reactions involved in cellular metabolism. The role of enzymes is to reduce the activation energies of biochemical reactions by forming complexes with its substrates. The lowering of activation energies favor an increase in the rates of biochemical reactions.
Enzyme deficiencies can often translate into life-threatening diseases. For example, a genetic abnormality resulting in the deficiency of the enzyme G6PD...
Enzyme deficiencies can often translate into life-threatening diseases. For example, a genetic abnormality resulting in the deficiency of the enzyme G6PD...
97.4K
01:24Biosynthesis in Bacteria
981
Biosynthesis in bacteria is a fundamental anabolic process that generates essential macromolecules, including proteins, nucleic acids, lipids, and polysaccharides. These macromolecules are critical for cellular growth, replication, and function. The process is tightly regulated and energetically linked to catabolic pathways to ensure optimal resource utilization.Biosynthetic pathways begin with precursor metabolites such as pyruvate, acetyl-CoA, and glucose-6-phosphate derived from glycolysis,...
981