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Related Concept Videos

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Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...
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The genomes of eukaryotes are punctuated by long stretches of sequence which do not code for proteins or RNAs. Although some of these regions do contain crucial regulatory sequences, the vast majority of this DNA serves no known function. Typically, these regions of the genome are the ones in which the fastest change, in evolutionary terms, is observed, because there is typically little to no selection pressure acting on these regions to preserve their sequences.
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Updated: Apr 10, 2026

Demonstration of the Sequence Alignment to Predict Across Species Susceptibility Tool for Rapid Assessment of Protein Conservation
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Sequence Display enables large-scale sequence-activity datasets for rapid protein evolution.

Linqi Cheng1, Xinzhe Zheng1, Shiyu Jason Jiang1

  • 1Department of Chemistry, Rice University, Houston, TX, USA.

Nature Biotechnology
|April 8, 2026
PubMed
Summary
This summary is machine-generated.

Sequence Display is a new platform for rapidly generating protein sequence-activity data. This accelerates the discovery of engineered proteins with novel functions for biological and medical applications.

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Area of Science:

  • Protein engineering
  • Synthetic biology
  • Biotechnology

Background:

  • Protein engineering is complex, often requiring extensive screening.
  • Mapping sequence-function relationships is crucial for protein design.

Purpose of the Study:

  • To introduce Sequence Display, a platform for high-throughput protein sequence-activity dataset generation.
  • To demonstrate the platform's utility in mapping protein activity landscapes.

Main Methods:

  • Sequence Display enables multiplexed assessment of individual variant activity in a single experiment.
  • Generated datasets for cytosine deaminase, uracil glycosylase inhibitor, aminoacyl-tRNA synthetase, and Cas9 nuclease.
  • Integrated Sequence Display data with protein language models.

Main Results:

  • Successfully mapped detailed sequence-function relationships for multiple proteins.
  • Discovered Cas9 variants with altered protospacer-adjacent motif recognition.
  • Evolved aminoacyl-tRNA synthetase variants for noncanonical amino acid recognition.

Conclusions:

  • Sequence Display is a powerful tool for accelerating protein engineering.
  • Enables rapid discovery of optimized proteins for diverse applications.