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  1. Home
  3. Circulating Horizontal Flow Bioreactor Using Tissue Engineering Scaffolds For Evaluating Prostate Cancer Metastasis To Bone.
  1. Home
  3. Circulating Horizontal Flow Bioreactor Using Tissue Engineering Scaffolds For Evaluating Prostate Cancer Metastasis To Bone.

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Circulating Horizontal Flow Bioreactor Using Tissue Engineering Scaffolds for Evaluating Prostate Cancer Metastasis

Preetham Ravi1, Shrinwanti Ghosh2, Sharad V Jaswandkar1

  • 1Department of Civil, Construction, and Environmental Engineering, North Dakota State University, Fargo, North Dakota, USA.

Journal of Biomedical Materials Research. Part B, Applied Biomaterials
|April 9, 2026

View abstract on PubMed

Summary
This summary is machine-generated.

This study developed a novel bioreactor to simulate prostate cancer metastasis to bone. The bioreactor models cell transitions and migration under fluid flow, aiding in understanding advanced prostate cancer progression.

Keywords:
bioreactorbone metastasiscirculating tumor cells (CTCs)interstitial fluid flowprostate cancer

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Area of Science:

  • Oncology
  • Biotechnology
  • Cell Biology

Background:

  • Prostate cancer mortality is linked to bone metastasis.
  • Metastasis involves epithelial to mesenchymal transition (EMT) and mesenchymal to epithelial transition (MET).
  • Hypoxia and interstitial fluid flow are key factors in cancer cell migration.

Purpose of the Study:

  • To fabricate a bioreactor for recapitulating prostate cancer cell EMT/MET cascade.
  • To evaluate the migration of hypoxic prostate cancer cells to bone under fluid flow.
  • To understand the role of hypoxia, integrins, and fluid flow in prostate cancer metastasis.

Main Methods:

  • Fabrication of a novel bioreactor with an injection port.
  • Assessment of αVβ3 integrin activation under hypoxia.
  • Evaluation of MET biomarkers (vimentin, E-cadherin) and angiogenic markers (VEGF, MMP-9, FAK) expression.
  • Recapitulation of cell migration using hanging drop spheroids and analysis of cell-fibroblast crosstalk.

    • Main Results:

    • Hypoxia activates αVβ3 integrins, enhancing cell attachment and growth.
    • Interstitial fluid flow influences the expression of αVβ3, E-cadherin, VEGF, MMP-9, and FAK.
    • Hypoxic PC3 cells show increased expression of angiogenic markers.
    • Distinct migratory patterns were observed, and cell-fibroblast crosstalk affects spheroid angiogenicity.

    Conclusions:

    • The developed bioreactor effectively mimics prostate cancer bone metastasis under interstitial fluid flow.
    • Understanding these migratory patterns can improve prediction of cancer progression.
    • This research aids in identifying therapies for advanced prostate cancer patients.