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Prognostic value and cellular function of hsa_circ_0001588 in breast cancer
Wei Wu1, Zichen Gao2, Sijing Gao3
1Breast and Thyroid Surgery, Dazhou Central Hospital, Dazhou, 635000 China.
Cytotechnology
|April 9, 2026
Summary
Hsa_circ_0001588 promotes breast cancer (BC) by regulating the miR-525-3p/KPNB1 axis. Its elevated expression correlates with poor BC prognosis, indicating potential as a diagnostic marker and therapeutic target.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Breast cancer (BC) is a prevalent malignancy in women.
- Circular RNAs (circRNAs) play a role in BC progression.
- Hsa_circ_0001588 is implicated in promoting BC through microRNA (miRNA) sponging.
Purpose of the Study:
- Investigate hsa_circ_0001588 expression in BC.
- Determine its functional role in BC progression.
- Elucidate its regulatory axis involving miR-525-3p and KPNB1.
Main Methods:
- RT-qPCR to assess hsa_circ_0001588 expression in clinical BC tissues and cell lines.
- MTT assays and Transwell systems to evaluate cell proliferation, migration, and invasion.
- Dual-luciferase reporter assays and RIP to confirm interactions within the hsa_circ_0001588/miR-525-3p/KPNB1 axis.
Main Results:
- Hsa_circ_0001588 was upregulated in BC tissues and cell lines, correlating with advanced TNM stage and poor prognosis.
- hsa_circ_0001588 depletion inhibited BC cell proliferation, migration, invasion, and epithelial-mesenchymal transition (EMT).
- hsa_circ_0001588 acts as a sponge for miR-525-3p, thereby releasing repression on KPNB1.
Conclusions:
- Hsa_circ_0001588 is a key regulator of BC malignancy via the miR-525-3p/KPNB1 pathway.
- Overexpression of hsa_circ_0001588 is linked to unfavorable prognosis.
- Hsa_circ_0001588 presents potential as a diagnostic biomarker and therapeutic target for breast cancer.
