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Related Concept Videos

Inhibitors of Viral Protein Synthesis01:30

Inhibitors of Viral Protein Synthesis

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Protein synthesis is indispensable for viral replication, as viruses lack the cellular machinery required for this process and must hijack the host's translational apparatus. In response, host cells deploy a critical innate immune defense involving interferons, specialized cytokines that play a central role in inhibiting viral propagation.Upon viral detection, infected cells release interferons that bind to receptors on adjacent uninfected cells, activating the JAK-STAT signaling pathway and...
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Inhibitors of Bacterial Protein Synthesis01:25

Inhibitors of Bacterial Protein Synthesis

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Aminoglycosides constitute a highly potent class of bactericidal antibiotics that exert their antimicrobial effects by targeting the bacterial ribosome, specifically disrupting protein synthesis. These polycationic molecules consist of amino-modified sugars linked via glycosidic bonds to an aminocyclitol core such as 2-deoxystreptamine or streptamine. Their strong positive charges facilitate tight binding to the negatively charged phosphate backbone of ribosomal RNA (rRNA), primarily at the 16S...
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Inhibitors of Bacterial DNA Synthesis01:28

Inhibitors of Bacterial DNA Synthesis

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Bacterial pathogens depend on precise and efficient DNA replication to sustain infection. Two type II topoisomerases—DNA gyrase and topoisomerase IV—are critical to this process, as they resolve DNA supercoiling and unlink chromosomes during replication. Fluoroquinolones, synthetic derivatives of quinolones, exploit this mechanism by stabilizing the transient DNA–enzyme cleavage complex, preventing strand religation, and causing lethal double-strand breaks. These...
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Eukaryotic Transcription Inhibitors01:52

Eukaryotic Transcription Inhibitors

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Certain biochemical processes, such as embryonic development and cell growth regulation, depend on the repression of specific genes. DNA binding proteins known as eukaryotic transcription inhibitors regulate the repression of gene expression in eukaryotes. The presence of these inhibitors at the required location and time in the cell is triggered by the presence of hormones and additional signals from other cells.
Eukaryotic transcription inhibitors usually contain two distinct domains, a...
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RNA Interference01:23

RNA Interference

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RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
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Related Experiment Video

Updated: Apr 10, 2026

Visualization of SARS-CoV-2 using Immuno RNA-Fluorescence In Situ Hybridization
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Lycorine Derivative Inhibits SARS-CoV-2 Replication by Reducing -1 Programmed Ribosomal Frameshifting via Targeting

Tingfu Du1, Ruixue Liu2, Xintian Zhang1

  • 1Institute of Medical Biology Chinese Academy of Medical Sciences & Peking Union Medical College Kunming China.

Medcomm
|April 9, 2026
PubMed
Summary
This summary is machine-generated.

A novel lycorine derivative, compound 7, shows potent antiviral activity against SARS-CoV-2 and its variants. It targets zinc-finger antiviral protein (ZAP-S) to inhibit viral replication, offering a promising therapeutic strategy.

Keywords:
SARS‐CoV‐2antiviral agentlycorine derivativeszinc‐finger antiviral protein–1 programmed ribosomal frameshifting

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Area of Science:

  • Virology
  • Drug Discovery
  • Molecular Biology

Background:

  • SARS-CoV-2 evolution necessitates broad-spectrum antiviral drugs.
  • Existing treatments face challenges due to viral immune evasion.
  • Lycorine derivatives present a potential avenue for antiviral development.

Purpose of the Study:

  • To synthesize and identify novel lycorine derivatives with antiviral activity.
  • To evaluate the efficacy of identified compounds against SARS-CoV-2 and its variants.
  • To elucidate the mechanism of action for promising antiviral candidates.

Main Methods:

  • Synthesis of lycorine derivatives and in vitro screening.
  • In vivo validation of antiviral efficacy in SARS-CoV-2 infected hamsters.
  • Mechanistic studies involving ZAP-S interaction, thermal shift assays, bio-layer interferometry, and mutagenesis.

Main Results:

  • Compound 7 demonstrated potent in vitro activity against SARS-CoV-2 and variants (Alpha, Beta, Delta, Omicron).
  • In vivo studies showed compound 7 significantly reduced viral loads and lung pathology in hamsters.
  • Compound 7 directly targets ZAP-S, enhancing its stability and inhibiting -1 programmed ribosomal frameshifting (-1PRF).

Conclusions:

  • Compound 7 is a ZAP-S-dependent antiviral agent effective against SARS-CoV-2.
  • Targeting ZAP-S to inhibit -1PRF represents a novel therapeutic strategy.
  • Compound 7 shows promise as a broad-spectrum antiviral drug for SARS-CoV-2 and emerging variants.