Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Development of Analytical Methods01:21

Development of Analytical Methods

3.1K
An analytical methodology can be divided into four sequential steps: technique, method, procedure, and protocol. A technique is a scientific principle that rationalizes a specific phenomenon through chemical measurements. Adapting a technique for analyzing a sample of interest is termed a method. The procedure outlines the directions for performing the analysis via an analytical method. The protocol is the detailed guidelines on the procedure, which should be strictly followed to obtain the...
3.1K
Data Validation01:15

Data Validation

3.6K
Method validation is a crucial process in analytical chemistry designed to confirm that a given method consistently produces reliable and high-quality results. This process is essential when a method is applied to different sample matrices or when procedural modifications are made, ensuring that the results meet acceptable standards across various applications.
Key parameters for method validation include:
3.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A dual-mode large language model assistant for on-surface reactions <i>via</i> fine-tuning and retrieval-augmented generation.

Chemical science·2026
Same author

Tunable Electronic Honeycomb and (Breathing) Kagome Lattices Through Molecular Orbital Design in 2D Metal-Organic Frameworks.

Angewandte Chemie (International ed. in English)·2026
Same author

FcγRIIb deficiency inhibits tumor development by attenuating the immunosuppressive phenotype of MDSCs.

Immunology letters·2026
Same author

Coordination preference of pyridine-based ligands on Ag(111).

Chemical communications (Cambridge, England)·2026
Same author

Deep learning drives autonomous molecular reactions with single-bond selectivity in tetra-brominated porphyrins on Au(111).

Nature communications·2026
Same author

LYZ Gene as a Novel Therapeutic Target and Diagnostic Biomarker in Glioblastoma: Insights from Multi-Omics Analysis and Functional Validation.

Biology·2026

Related Experiment Video

Updated: Apr 10, 2026

Identification of Pharmaceuticals in The Aquatic Environment Using HPLC-ESI-Q-TOF-MS and Elimination of Erythromycin Through Photo-Induced Degradation
05:46

Identification of Pharmaceuticals in The Aquatic Environment Using HPLC-ESI-Q-TOF-MS and Elimination of Erythromycin Through Photo-Induced Degradation

Published on: August 1, 2018

13.7K

Development and validation of a novel analytical method for related substances of resmetirom and identification of

Jisu Qin1,2, Qunfeng Luo3, Wenyi Wu4

  • 1Department of Pharmacy, Affiliated Nantong Hospital of Shanghai University (The Sixth People's Hospital of Nantong), Nantong, China.

Frontiers in Chemistry
|April 9, 2026
PubMed
Summary

A new RP-HPLC method accurately detects related substances in resmetirom, a NASH medication. This validated method ensures drug quality and patient safety by identifying impurities, including a novel degradation product.

Keywords:
method developmentmethod validationnovel productsrelated substancesresmetirom

More Related Videos

GC-based Detection of Aldononitrile Acetate Derivatized Glucosamine and Muramic Acid for Microbial Residue Determination in Soil
11:06

GC-based Detection of Aldononitrile Acetate Derivatized Glucosamine and Muramic Acid for Microbial Residue Determination in Soil

Published on: May 19, 2012

17.8K
Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS
11:00

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS

Published on: May 20, 2013

23.6K

Related Experiment Videos

Last Updated: Apr 10, 2026

Identification of Pharmaceuticals in The Aquatic Environment Using HPLC-ESI-Q-TOF-MS and Elimination of Erythromycin Through Photo-Induced Degradation
05:46

Identification of Pharmaceuticals in The Aquatic Environment Using HPLC-ESI-Q-TOF-MS and Elimination of Erythromycin Through Photo-Induced Degradation

Published on: August 1, 2018

13.7K
GC-based Detection of Aldononitrile Acetate Derivatized Glucosamine and Muramic Acid for Microbial Residue Determination in Soil
11:06

GC-based Detection of Aldononitrile Acetate Derivatized Glucosamine and Muramic Acid for Microbial Residue Determination in Soil

Published on: May 19, 2012

17.8K
Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS
11:00

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS

Published on: May 20, 2013

23.6K

Area of Science:

  • Pharmaceutical Analysis
  • Chromatography
  • Drug Quality Control

Background:

  • Resmetirom is indicated for nonalcoholic steatohepatitis (NASH) with liver fibrosis.
  • Related substances critically affect API safety and efficacy.
  • Accurate quantification of resmetirom impurities is essential.

Purpose of the Study:

  • To develop and validate a reverse-phase high-performance liquid chromatography (RP-HPLC) method.
  • To enable the separation and detection of resmetirom and its related substances.
  • To support the quality control of resmetirom drug products.

Main Methods:

  • Utilized an Agilent 5 HC C18 column with gradient elution.
  • Employed a mobile phase of H2O/ACN with TFA.
  • Validated the method per ICH guidelines for specificity, stability, linearity, precision, and robustness.

Main Results:

  • Achieved effective separation of resmetirom and impurities within 45 minutes.
  • Identified and elucidated the structure of a novel degradation product (imp-A) using NMR and HRMS.
  • Demonstrated method specificity, stability, linearity, precision, repeatability, and robustness.

Conclusions:

  • The developed RP-HPLC method reliably separates and detects resmetirom and its related substances.
  • The method is simple, efficient, and provides dependable results for quality control.
  • This analytical tool enhances the safety and efficacy of resmetirom formulations.