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Enhanced DNA Data Storage Stability and Amplification Efficiency Using Extreme Thermostable Single-Stranded
Yihao Huang1, Xinxin Ran1, Meizhen Jiang1
1The MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, Department of Chemical Biology, College of Chemistry and Chemical Engineering, State Key Laboratory of Vaccines for Infectious Diseases, Fujian Key Laboratory of Chemical Biology (Xiamen University), Xiang An Biomedicine Laboratory, Xiamen University, Xiamen, Fujian, China.
Small Methods
|April 9, 2026
Summary
Researchers developed a new DNA data storage method using extreme thermostable single-strand DNA-binding proteins (ET SSB). This stabilizes DNA, preventing degradation and improving polymerase chain reaction (PCR) efficiency for reliable data retrieval.
Area of Science:
- Biotechnology
- Molecular Biology
- Bioinformatics
Background:
- DNA offers high storage density and durability for digital information.
- Long-term stability and protection from degradation are key challenges in DNA data storage.
Purpose of the Study:
- To present a novel method for enhancing DNA data storage stability and retrieval efficiency.
- To investigate the utility of extreme thermostable single-strand DNA-binding proteins (ET SSB) in DNA data storage.
Main Methods:
- Utilized extreme thermostable single-strand DNA-binding proteins (ET SSB) to stabilize single-stranded DNA (ssDNA).
- Employed microdroplet polymerase chain reaction (PCR) for DNA amplification and information decoding.
Main Results:
- ET SSB protected DNA from environmental degradation and contamination.
- ET SSB preserved ssDNA conformation, enhancing PCR efficiency.
- Achieved amplification and decoding with as few as 10 DNA template molecules.
Conclusions:
- ET SSB significantly enhances the stability and retrieval efficiency of DNA data storage.
- This method advances the practical application of DNA as a stable and efficient information storage medium.
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