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Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules
Published on: April 12, 2019
Enhanced DNA Data Storage Stability and Amplification Efficiency Using Extreme Thermostable Single-Stranded
Yihao Huang1, Xinxin Ran1, Meizhen Jiang1
1The MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, Department of Chemical Biology, College of Chemistry and Chemical Engineering, State Key Laboratory of Vaccines for Infectious Diseases, Fujian Key Laboratory of Chemical Biology (Xiamen University), Xiang An Biomedicine Laboratory, Xiamen University, Xiamen, Fujian, China.
Abstract:
Owing to its durability, replicability, and high storage density, DNA has emerged as a highly promising medium for digital information storage. However, ensuring long-term storage stability remains a significant challenge in the design of DNA-based storage systems. In this study, we present a method for DNA data storage that utilizes extreme thermostable single-strand DNA-binding proteins (ET SSB), which bind to and stabilize single-stranded DNA (ssDNA), protecting it from environmental degradation and contamination. Additionally, ET SSB preserves the template's single-stranded conformation, enhancing polymerase extension and promoting the efficiency of the polymerase chain reaction (PCR). When combined with microdroplet PCR, this method enables amplification and information decoding with as few as 10 DNA template molecules. This dual functionality not only highlights the potential value of ET SSB but also contributes to advancing the practical use of DNA as a stable and efficient medium for information storage.
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