Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cell Polarization by Rho Proteins01:21

Cell Polarization by Rho Proteins

4.1K
Cell polarity is the asymmetric distribution of cellular and membrane components, making one side of the cell different from the other. This polarity is essential to many processes such as embryogenesis, axon migration, glucose transport across epithelial cells, and directional cell migration. A migrating cell responds to intracellular or extracellular signals via molecular cascades that reorganize the actin cytoskeleton to establish this polarity. In these cells, the Rho family proteins Cdc42,...
4.1K
Small GTPases - Ras and Rho01:24

Small GTPases - Ras and Rho

5.7K
Ras and Rho are small monomeric GTPases that act downstream of receptor tyrosine kinase (RTK) and regulate various cellular processes. These GTPases switch between active and inactive states by binding to guanine nucleotides.
Three regulatory proteins control their activity:
5.7K
Mechanism of Lamellipodia Formation01:31

Mechanism of Lamellipodia Formation

4.0K
Cells migrating in response to external stimuli form lamellipodia, which are thin membrane protrusions supported by a mesh of linked, branched, or unbranched actin filaments. These actin filaments interact with myosin motor proteins, creating the dynamic actomyosin complex within the cytoskeleton. Contractility, or the ability to generate contractile stress, is inherent to the actomyosin complex. It helps cells detect the stiffness of the surrounding ECM and exert contractile force for...
4.0K
Cytoskeletal Coordination in Cell Migration01:32

Cytoskeletal Coordination in Cell Migration

5.8K
A migrating cell changes its shape during the cyclic events of attachment and detachment from the substratum and repositions the cell organelles correspondingly. These complex events are orchestrated by the dynamic cytoskeletal network comprising actin filaments, intermediate filaments, and microtubules. Cytoskeletal crosstalk — the direct and indirect communication between the different components — is crucial for this coordination. Direct communication involves various linker...
5.8K
Cell Migration01:09

Cell Migration

19.2K
Cell migration, the process by which cells move from one location to another, is essential for the proper development and viability of organisms throughout their life. When cells are not able to migrate properly to their ordained locations, various disorders may occur. For example, disruption in cell migration causes chronic inflammatory diseases such as arthritis.
19.2K
Cell Migration01:19

Cell Migration

7.5K
Cell migration is a process by which the cells move from one location to another, playing an essential role in embryological development, repair and regeneration, immune response, and metastasis. Cells migrate in response to chemical or mechanical signals generated by specific organs or tissues. The overall mechanism includes three steps - polarization, protrusion, and release. Polarization involves the formation of a distinct cell front and rear, which determines the direction of movement.
7.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Integrated Transcriptomic and Proteomic Analyses Reveal CsrA-Mediated Regulation of Virulence and Metabolism in <i>Vibrio alginolyticus</i>.

Microorganisms·2025
Same author

Capture, mutual inhibition and release mechanism for aPKC-Par6 and its multisite polarity substrate Lgl.

Nature structural & molecular biology·2025
Same author

The Dystrophin-Dystroglycan complex ensures cytokinesis efficiency in Drosophila epithelia.

EMBO reports·2024
Same author

Identification of the global regulatory roles of RraA via the integrative transcriptome and proteome in <i>Vibrio alginolyticus</i>.

mSphere·2024
Same author

Basal actomyosin pulses expand epithelium coordinating cell flattening and tissue elongation.

Nature communications·2024
Same author

Kinesin-1 patterns Par-1 and Rho signaling at the cortex of syncytial embryos of Drosophila.

The Journal of cell biology·2023

Related Experiment Video

Updated: Apr 10, 2026

Author Spotlight: Optogenetic Inhibition of Rho1-Mediated Actomyosin Contractility Coupled with Measurement of Epithelial Tension in Drosophila Embryos
12:35

Author Spotlight: Optogenetic Inhibition of Rho1-Mediated Actomyosin Contractility Coupled with Measurement of Epithelial Tension in Drosophila Embryos

Published on: April 14, 2023

2.0K

Spatial inhibition of RhoA by RhoGAP15B promotes protrusive activity during collective migration.

Vítor Yang1,2, Cristian Marchant1,2, Bing Liu3

  • 1IBMC - Instituto de Biologia Molecular e Celular, Universidade do Porto , Porto, Portugal.

The Journal of Cell Biology
|April 9, 2026
PubMed
Summary

This study identifies RhoGAP15B as a key regulator of collective cell migration. It acts as a localized inhibitor of RhoA activity, controlling cell shape and movement dynamics.

More Related Videos

RhoC GTPase Activation Assay
09:58

RhoC GTPase Activation Assay

Published on: August 22, 2010

13.2K
Analyzing In Vivo Cell Migration using Cell Transplantations and Time-lapse Imaging in Zebrafish Embryos
11:39

Analyzing In Vivo Cell Migration using Cell Transplantations and Time-lapse Imaging in Zebrafish Embryos

Published on: April 29, 2016

8.3K

Related Experiment Videos

Last Updated: Apr 10, 2026

Author Spotlight: Optogenetic Inhibition of Rho1-Mediated Actomyosin Contractility Coupled with Measurement of Epithelial Tension in Drosophila Embryos
12:35

Author Spotlight: Optogenetic Inhibition of Rho1-Mediated Actomyosin Contractility Coupled with Measurement of Epithelial Tension in Drosophila Embryos

Published on: April 14, 2023

2.0K
RhoC GTPase Activation Assay
09:58

RhoC GTPase Activation Assay

Published on: August 22, 2010

13.2K
Analyzing In Vivo Cell Migration using Cell Transplantations and Time-lapse Imaging in Zebrafish Embryos
11:39

Analyzing In Vivo Cell Migration using Cell Transplantations and Time-lapse Imaging in Zebrafish Embryos

Published on: April 29, 2016

8.3K

Area of Science:

  • Cell Biology
  • Developmental Biology
  • Molecular Biology

Background:

  • Rho family GTPases (RhoA, Rac1, Cdc42) are crucial for collective cell migration.
  • The spatial and temporal coordination of Rho GTPase activators (RhoGEFs) and inhibitors (RhoGAPs) remains poorly understood.

Purpose of the Study:

  • To systematically characterize the localization of Drosophila RhoGEFs and RhoGAPs in border cells.
  • To identify the functional roles of these regulators in collective cell migration.

Main Methods:

  • Utilized GFP knock-in lines to map RhoGEF and RhoGAP localization in Drosophila border cells.
  • Employed RNAi screening combined with GFP validation to assess the functional significance of RhoGEFs/GAPs.
  • Investigated the role of RhoGAP15B in regulating RhoA activity and cell migration.

Main Results:

  • Identified RhoGAP15B as a specific inhibitor of RhoA activity localized to the border cell cortex.
  • Demonstrated that RhoGAP15B restrains actomyosin contractility at the leading cell front, promoting cell protrusion.
  • Showed RhoGAP15B regulates border cell cluster morphology during collective migration.

Conclusions:

  • RhoGAP15B is a critical spatial regulator of RhoA activity during collective cell migration.
  • Patterned RhoGAP and RhoGEF activity is essential for coordinating cortical contractility and protrusion dynamics.