Related Experiment Video
Updated: Apr 11, 2026

Preparation of Gynura bicolor DC samples for High-Resolution Tandem Mass Spectrometry
Published on: February 2, 2024
Chemical profiling and quality marker constituents screening of Bupleurum scorzonerifolium based on GC-MS and
Yakun Xiao1, Xinlong Zhu1, Wenxue Wang1
1School of Pharmacy, Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang, China.
Abstract:
The root of Bupleurum scorzonerifolium (BSR) is a commonly used traditional Chinese medicine for treating fever, hepatitis, depression, etc. Currently, cultivated BSR has been widely used in clinical practice. However, its chemical components have not been systematically studied, and quality indicators remain unclear. In this study, the chemical composition of 13 batches of cultivated BSR was analysed using GC-MS and UPLC-Q-TOF/MS. A total of 89 volatile components and 68 non-volatile components were identified. Through multivariate statistical analysis, 11 chemical components with VIP > 1, p < 0.05, FC > 1 or FC < 0.5 were selected as potential markers. The quantitative methods for 3 volatile components (decyl acetate, dodecanal, and 1-dodecanol) and 3 saikosaponins (saikosaponins a, b2, and c) were established using GC-MS and HPLC, respectively. These six components can serve as indicators for assessing the quality of cultivated BSR. These findings provide a theoretical basis for establishing a quality evaluation system for cultivated BSR and guiding its clinical application.
More Related Videos
07:29HPLC Coupled with Chemical Fingerprinting for Multi-Pattern Recognition for Identifying the Authenticity of Clematidis Armandii Caulis
Published on: November 11, 2022
05:29Profiling Volatile Compounds in Blackcurrant Fruit using Headspace Solid-Phase Microextraction Coupled to Gas Chromatography-Mass Spectrometry
Published on: June 9, 2021
Related Concept Videos
Gas Chromatography–Mass Spectrometry (GC–MS)
A gas chromatograph consists of a long, narrow capillary column with a polysiloxane coating on the inner wall....
Mass Spectrometry: Complex Analysis
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...