Multiplexed nanophotonic sensor arrays for time-resolved biomolecular analysis

Area of Science:

• Biophotonics
• Biosensing Technology
• Molecular Interactions

Background:

• Complex diseases involve intricate biomolecular networks.
• Current analytical methods often limit simultaneous interaction measurements.
• Need for high-throughput, sensitive biomolecular analysis exists.

Purpose of the Study:

• To develop a multiplexed, label-free biosensor array for biomolecular kinetics.
• To enable simultaneous analysis of hundreds of sensing sites.
• To facilitate high-throughput biomolecular profiling.

Main Methods:

• Fabrication of a chirped guided-mode resonance (cGMR) photonic biosensor array using CMOS process.
• Integration of 322 photonic sensors on a single chip.
• Time-resolved, simultaneous measurements using a conventional CMOS camera.

Main Results:

• Demonstrated real-time binding of antibodies, aptamers, and glycopolymers.
• Revealed distributions of binding responses due to surface heterogeneity and multivalency.
• Resolved lectin-glycopolymer binding kinetics with sub-micromolar dissociation constants.

Conclusions:

• The cGMR array offers a robust platform for high-throughput biomolecular profiling.
• Scalable CMOS fabrication combined with parallel interrogation enhances biosensing capabilities.
• The technology shows promise for point-of-care diagnostics and drug discovery.