Evaluation of Zanthoxylum bungeanum sprouts amino acids profile by capillary electrophoresis and principal component...

Zanthoxylum bungeanum sprouts (ZBS) rich nutritional component have long history of edible and favored by consumers. In this study, a facile, rapid method was proposed for assay the amino acids profile in ZBS based capillary electrophoresis. The effect of the concentration of borax, α-cyclodextrin (α-CD), and the pH of the separation background electrolyte (BGE) were optimized. Under optimal conditions, baseline separation of 13 AAs was achieved, with a linear range of 20-300 μmol/L and a linear correlation coefficient (R²) 0.9837-0.9994. The RSD of the migration time and peak area were ranged in 2.5-4.8% and 1.6-5.0%, respectively the limit of detection (LOD) was 3.6-11.7 μmol/L. Phenylalanine, histidine, glycine, threonine, serine and alanine were commonly found in 35 ZBS. The content of the amino acids in ZBS ranged from 1.57 to 12.82 mg/g, in which the glycine content occupied about 50%. This work demonstrated promising potential for the quality study of ZBS.