Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Responses to Heat and Cold Stress02:45

Responses to Heat and Cold Stress

15.8K
Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes.
15.8K
Cell Signaling in Plants01:25

Cell Signaling in Plants

7.1K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
7.1K
Morphogenesis02:19

Morphogenesis

30.9K
Plant morphogenesis—the development of a plant’s form and structure—involves several overlapping developmental processes, including growth and cell differentiation. Precursor cells differentiate into specific cell types, which are organized into the tissues and organ systems that make up the functional plant.
30.9K
Responses to Salt Stress02:02

Responses to Salt Stress

15.1K
Salt stress—which can be triggered by high salt concentrations in a plant’s environment—can significantly affect plant growth and crop production by influencing photosynthesis and the absorption of water and nutrients.
15.1K
Plant Cell Wall02:43

Plant Cell Wall

61.9K
The plant cell wall gives plant cells shape, support, and protection. As a cell matures, its cell wall specializes according to the cell type. For example, the parenchyma cells of leaves possess only a thin, primary cell wall.
61.9K
Plant Cell Wall01:07

Plant Cell Wall

9.0K
Plant cells have a cell wall, a rigid outer covering that protects the cell and provides shape and support. During cell division, a mixture of enzymes, proteins, and glucose molecules is transported via vesicles to the center of the cell. These vesicles continuously fuse and build a cell plate between the dividing cells. As the cell plate matures, new polysaccharides are added to it to form the cell walls of the daughter cells. The predominant polysaccharide in the cell wall is cellulose, made...
9.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Plant RABC1 GTPase Coordinates with Exocyst Component SEC5A in Regulating ER-phagy under Endoplasmic Reticulum Stress.

The Plant cell·2026
Same author

Functional Overview of Plant Genes Essential for Arbuscular Mycorrhizal Symbiosis.

Genes·2026
Same author

DMI3 autophosphorylation at the C-terminus of its calmodulin-binding domain dismantles CaM-DMI3-IPD3 to initiate root nodulation.

Plant physiology·2026
Same author

Vacuolar sorting receptors regulates autophagic trafficking of pathogenic effectors in plant immunity.

Autophagy·2026
Same author

Small peptide from extracellular leucine-rich repeat domain protein 2 modulates cadmium tolerance through regulating photosynthesis and lignin content in rice.

International journal of biological macromolecules·2026
Same author

The Solanaceae-conserved peptide NbSolP9 enhances tobacco resistance to Botrytis cinerea by regulating nicotine metabolism.

Plant physiology and biochemistry : PPB·2026

Related Experiment Video

Updated: Apr 11, 2026

Imaging Spatial Reorganization of a MAPK Signaling Pathway Using the Tobacco Transient Expression System
08:54

Imaging Spatial Reorganization of a MAPK Signaling Pathway Using the Tobacco Transient Expression System

Published on: March 20, 2016

10.4K

Mapping plant cell-type-specific responses to environmental stresses.

Muhammad Ali1, Zhengjia Wang1, Qiuyue Guo2

  • 1State Key Laboratory for Development and Utilization of Forest Food Resources, Zhejiang A&F University, Hangzhou 311300, China; Zhejiang Key Laboratory of Non-wood Forest Products Quality Regulation and Processing Utilization, Zhejiang A&F University, Hangzhou 311300, China.

Trends in Plant Science
|April 9, 2026
PubMed
Summary

Understanding plant stress responses requires advanced techniques. Single-cell and spatial transcriptomics reveal cell-type-specific details crucial for climate-smart agriculture.

Keywords:
biotic and abiotic stressesplant responsesplant–pathogen interactionsscRNA-seqspatial transcriptomics

More Related Videos

Investigating Tissue- and Organ-specific Phytochrome Responses using FACS-assisted Cell-type Specific Expression Profiling in Arabidopsis thaliana
10:10

Investigating Tissue- and Organ-specific Phytochrome Responses using FACS-assisted Cell-type Specific Expression Profiling in Arabidopsis thaliana

Published on: May 29, 2010

16.4K
Bacterial Leaf Infiltration Assay for Fine Characterization of Plant Defense Responses using the Arabidopsis thaliana-Pseudomonas syringae Pathosystem
11:50

Bacterial Leaf Infiltration Assay for Fine Characterization of Plant Defense Responses using the Arabidopsis thaliana-Pseudomonas syringae Pathosystem

Published on: October 1, 2015

23.1K

Related Experiment Videos

Last Updated: Apr 11, 2026

Imaging Spatial Reorganization of a MAPK Signaling Pathway Using the Tobacco Transient Expression System
08:54

Imaging Spatial Reorganization of a MAPK Signaling Pathway Using the Tobacco Transient Expression System

Published on: March 20, 2016

10.4K
Investigating Tissue- and Organ-specific Phytochrome Responses using FACS-assisted Cell-type Specific Expression Profiling in Arabidopsis thaliana
10:10

Investigating Tissue- and Organ-specific Phytochrome Responses using FACS-assisted Cell-type Specific Expression Profiling in Arabidopsis thaliana

Published on: May 29, 2010

16.4K
Bacterial Leaf Infiltration Assay for Fine Characterization of Plant Defense Responses using the Arabidopsis thaliana-Pseudomonas syringae Pathosystem
11:50

Bacterial Leaf Infiltration Assay for Fine Characterization of Plant Defense Responses using the Arabidopsis thaliana-Pseudomonas syringae Pathosystem

Published on: October 1, 2015

23.1K

Area of Science:

  • Plant Biology
  • Genomics
  • Climate Change Adaptation

Background:

  • Plants face increasing biotic and abiotic stresses due to climate change.
  • Traditional methods like bulk RNA sequencing obscure crucial cell-specific stress responses.
  • Understanding cellular heterogeneity is vital for plant survival and productivity.

Purpose of the Study:

  • To review advances in single-cell/nucleus RNA sequencing and spatial transcriptomics for plant stress studies.
  • To highlight the potential of these technologies in dissecting cell-type-specific stress responses.
  • To advocate for the adoption of these methods in plant science.

Main Methods:

  • Single-cell RNA sequencing (scRNA-seq)
  • Single-nucleus RNA sequencing (snRNA-seq)
  • Spatial transcriptomics

Main Results:

  • These advanced techniques enable high-resolution mapping of plant transcriptional programs.
  • scRNA-seq and snRNA-seq reveal cell-type-specific responses to stresses.
  • Spatial transcriptomics provides spatial context to cellular responses.

Conclusions:

  • Single-cell and spatial transcriptomics bridge gaps in understanding plant stress biology.
  • These methods are essential for detailed analysis of plant responses to environmental challenges.
  • Expanding their use can accelerate the development of climate-smart agriculture.