Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Patterns of Response to Immune Checkpoint Inhibitors in Sarcomas With High Tumor Burden and/or Mismatch Repair Deficiency.

JCO precision oncology·2026
Same author

Genetic resonance in the p53 signaling network.

Cell systems·2026
Same author

DNA damage checkpoints balance a tradeoff between diploid- and polyploid-derived arrest failures.

Cell reports·2025
Same author

Decoding the Central Dogma: Quantitative Insights into Transcription and Translation Dynamics in the p53-Mediated DNA Damage Response.

Journal of molecular biology·2025
Same author

Comparison of <i>TP53</i> mutations in myelodysplasia and acute leukemia suggests divergent roles in initiation and progression.

Blood neoplasia·2025
Same author

Detection of TP53 mutations by immunohistochemistry in acute myeloid leukemia varies with interpreter expertise and mutation status.

American journal of clinical pathology·2025
Same journal

The evolution of the United States nucleic acid screening policies.

Frontiers in bioengineering and biotechnology·2026
Same journal

Experimental study on antler powder/chitosan/β-glycerophosphate/polyvinyl alcohol composite platelet-rich fibrin scaffold for repairing periodontal bone defects in rabbits.

Frontiers in bioengineering and biotechnology·2026
Same journal

Integrating artificial intelligence and conventional approaches in sugarcane bagasse biorefineries: a review towards a circular bioeconomy.

Frontiers in bioengineering and biotechnology·2026
Same journal

Germanium-embedded bioactive fabric reduces bacterial bioburden and modulates fibroblast and macrophage behavior <i>in vitro</i>.

Frontiers in bioengineering and biotechnology·2026
Same journal

Sequentially coupled musculoskeletal multibody and finite element simulation for biomechanical modeling of the human masticatory system.

Frontiers in bioengineering and biotechnology·2026
Same journal

Elliptical defects create a more adverse biomechanical environment than circular defects in osteochondral lesion of the talus: a finite element analysis.

Frontiers in bioengineering and biotechnology·2026
See all related articles

Related Experiment Video

Updated: Apr 11, 2026

Author Spotlight: Radiotherapy and Clonogenic Assays for Advancing Cancer Research and Personalized Medicine
05:39

Author Spotlight: Radiotherapy and Clonogenic Assays for Advancing Cancer Research and Personalized Medicine

Published on: April 5, 2024

1.4K

Quantifying single-cell responses to irradiation in 3D.

Joshua François1, Alina Simerzin1, Ashwini Jambhekar1

  • 1Department of Systems Biology, Blavantik Institute, Harvard Medical School, Boston, MA, United States.

Frontiers in Bioengineering and Biotechnology
|April 10, 2026
PubMed
Summary
This summary is machine-generated.

This study developed advanced tools to track cell responses in 3D cultures, revealing that cell signaling kinetics differ significantly in spheroids compared to 2D systems after irradiation.

Keywords:
3Dimagingmatrigel assaymicroscopyp21 (CDKN1A)spheroids

More Related Videos

Growth and Characterization of Irradiated Organoids from Mammary Glands
09:49

Growth and Characterization of Irradiated Organoids from Mammary Glands

Published on: May 3, 2019

8.7K
Generation of High-Throughput Three-Dimensional Tumor Spheroids for Drug Screening
05:54

Generation of High-Throughput Three-Dimensional Tumor Spheroids for Drug Screening

Published on: September 5, 2018

9.2K

Related Experiment Videos

Last Updated: Apr 11, 2026

Author Spotlight: Radiotherapy and Clonogenic Assays for Advancing Cancer Research and Personalized Medicine
05:39

Author Spotlight: Radiotherapy and Clonogenic Assays for Advancing Cancer Research and Personalized Medicine

Published on: April 5, 2024

1.4K
Growth and Characterization of Irradiated Organoids from Mammary Glands
09:49

Growth and Characterization of Irradiated Organoids from Mammary Glands

Published on: May 3, 2019

8.7K
Generation of High-Throughput Three-Dimensional Tumor Spheroids for Drug Screening
05:54

Generation of High-Throughput Three-Dimensional Tumor Spheroids for Drug Screening

Published on: September 5, 2018

9.2K

Area of Science:

  • Cell Biology
  • Biophysics
  • Cancer Research

Background:

  • Three-dimensional (3D) cell cultures better mimic physiological conditions than 2D systems, enabling investigation of cell-cell and cell-extracellular matrix interactions.
  • Quantifying cellular signaling dynamics in 3D environments is challenging due to imaging and computational limitations.

Purpose of the Study:

  • To develop and validate experimental and computational tools for tracking individual cell responses in 3D.
  • To investigate the dynamics of p21 activation in human breast cancer cells following irradiation in 3D cultures.

Main Methods:

  • Developed a dual-Matrigel assay to embed individual cells and spheroids.
  • Utilized light sheet fluorescence microscopy (LSFM) for high-resolution imaging.
  • Created computational pipelines for quantitative analysis of p21 dynamics.

Main Results:

  • Dispersed cells showed a gradual increase in p21-positive cells post-irradiation, while spheroids exhibited transient decrease followed by a delayed rise.
  • p21 intensity remained constant, suggesting regulation of transcription probability rather than magnitude.
  • Spheroids displayed slower p21 induction kinetics compared to 2D and 3D single-cell cultures.

Conclusions:

  • The developed platform enables dissection of signaling dynamics in physiologically relevant 3D contexts.
  • Differences in p21 induction kinetics in spheroids highlight the impact of 3D architecture and cell-extracellular matrix interactions.
  • The framework can be extended to study drug effects on complex multicellular structures.