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Expanding the C. elegans toolkit with gonad explants.

Réda M Zellag1,2, Eric Cheng1, Abigail R Gerhold1

  • 1Department of Biology, McGill University, 1205 avenue Docteur Penfield, Montréal, QC, H2A 1B1, Canada.

Biorxiv : the Preprint Server for Biology
|April 10, 2026
PubMed
Summary

Extruded C. elegans gonads serve as a novel explant system for studying germline development. This system allows live-cell imaging and drug treatment to investigate complex developmental mechanisms in vivo.

Keywords:
Caenorhabditis elegansdrug treatmentex vivo culturegonad developmentmitosis and meiosistissue explant

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Area of Science:

  • Developmental Biology
  • Cell Biology
  • Genetics

Background:

  • Animal development involves complex spatiotemporal coordination of numerous pathways.
  • Tissue explants offer simplified models for investigating dynamic biological events.
  • Investigating germline development mechanisms requires robust experimental systems.

Purpose of the Study:

  • To establish and validate the use of extruded C. elegans gonads as a viable tissue explant system.
  • To demonstrate the utility of this system for live-cell imaging and mechanistic studies of germline development.
  • To investigate the responsiveness of gonad explants to pharmacological treatments.

Main Methods:

  • Extrusion of C. elegans gonads to create tissue explants.
  • Live-cell imaging to observe cellular processes within explants.
  • Treatment of explants with nocodazole, a microtubule depolymerizing drug.

Main Results:

  • C. elegans gonad explants retain key in vivo tissue properties, including mitosis, meiosis, apoptosis, and gametogenesis.
  • The explant system supports live-cell imaging of essential developmental events.
  • Gonad explants exhibit acute responses to nocodazole treatment, indicating preserved cellular functions.

Conclusions:

  • Extruded C. elegans gonads represent a valuable new model system for studying germline development.
  • This explant system enables the combination of live-cell imaging and acute drug treatment to decipher developmental mechanisms.
  • The findings open new avenues for mechanistic investigations into C. elegans germline biology.