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Lysinibacillus macroides stimulates systemic resistance against Potyvirus papayanuli in cucumber
Mohsen M Elsharkawy1, Faisal Ay Alzahrani2
1Department of Agricultural Botany, Faculty of Agriculture, Kafrelsheikh University, Kafr Elsheikh, Egypt.
Introduction:
New and potentially useful rhizobacterial isolates are the focus of the most ongoing research into phytoviruses management because of their ability to inhibit viral replication.
Methods:
We investigated the efficacy of a newly-isolated strain of Lysinibacillus macroides in controlling Potyvirus papayanuli (papaya ring spot virus, PRSV) in cucumber plants.
Results And Discussion:
Significant changes were observed between the treatment and control groups. For instance, cucumber plants were shown to have less severe viral symptoms after being pre-treated with L. macroides and then inoculated with PRSV. Upon application of L. macroides soil drenching (SD) treatment, the disease severity rate decreased significantly by 78.3% and 71.5% at 2- and 3- weeks post-infection (WPI), respectively. Plants treated with L. macroides had a lower PRSV titer using ELISA. Moreover, in PRSV-infected cucumbers, L. macroides treatments significantly increased antioxidant enzymes production and defense gene expressions as compared to the control treatment. Therefore, it is possible that L. macroides induces systemic resistance in cucumber plants and adversely impacts PRSV development. As a sustainable and eco-friendly approach for PRSV management, our study is the first report on the antiphytoviral activity of L. macroides against PRSV.
Insights
Lysinibacillus macroides effectively manages papaya ring spot virus (PRSV) in cucumbers. This beneficial bacterium reduces disease severity and viral load, offering a sustainable approach to plant disease control.
Area of Science:
- Plant Pathology
- Microbiology
- Agricultural Science
Background:
- Rhizobacteria show potential in managing phytoviruses by inhibiting viral replication.
- Sustainable and eco-friendly approaches are crucial for effective plant disease management.
Purpose of the Study:
- To evaluate the efficacy of Lysinibacillus macroides (L. macroides) in controlling papaya ring spot virus (PRSV) in cucumber plants.
- To investigate the impact of L. macroides on viral symptoms, viral load, and plant defense mechanisms.
Main Methods:
- A newly-isolated strain of L. macroides was used for soil drenching (SD) treatment on cucumber plants.
- Plants were inoculated with PRSV, and disease severity, viral titer (ELISA), antioxidant enzyme activity, and defense gene expression were assessed.
Main Results:
- L. macroides soil drenching significantly reduced PRSV disease severity by 78.3% and 71.5% at 2 and 3 weeks post-infection, respectively.
- ELISA confirmed a lower PRSV titer in L. macroides-treated plants.
- Treatment with L. macroides increased antioxidant enzyme production and defense gene expression in infected cucumber plants.
Conclusions:
- L. macroides demonstrates significant antiphytoviral activity against PRSV in cucumbers.
- The bacterium likely induces systemic resistance in plants, adversely affecting PRSV development.
- L. macroides represents a novel, sustainable, and eco-friendly strategy for PRSV management.
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