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BGP-15 Mitigates Oxidative Stress and Mitochondrial Dysfunction in In Vitro Matured Oocytes From Type 1 Diabetic Mice
Luyao Wang1, Lin Yin1, Yangyang Li1
1Department of Obstetrics and Gynecology, Center for Reproductive Medicine, the Fourth Affiliated Hospital of School of Medicine, and International School of Medicine, International Institutes of Medicine, Zhejiang University, Yiwu, China.
Supplementing with BGP-15 in vitro maturation (IVM) medium improves oocyte quality in diabetic mice by reducing oxidative stress and mitochondrial damage. This finding offers a potential strategy to enhance reproductive outcomes for women with diabetes.
Area of Science:
- Reproductive Biology
- Endocrinology
- Mitochondrial Medicine
Background:
- Diabetes mellitus negatively impacts oocyte quality, leading to impaired embryo development and pregnancy outcomes.
- Effective strategies to improve oocyte quality in diabetic individuals remain largely undetermined.
- Maternal diabetes poses a significant risk to reproductive health and fertility.
Purpose of the Study:
- To investigate the potential of BGP-15 as a therapeutic agent to improve oocyte quality in a mouse model of diabetes.
- To elucidate the mechanisms by which BGP-15 exerts its protective effects on diabetic oocytes.
- To assess the impact of BGP-15 on oocyte maturation rates and cellular health.
Main Methods:
- In vitro maturation (IVM) of oocytes from diabetic and control mice with and without BGP-15 supplementation.
- Assessment of oocyte maturation rates, oxidative stress markers, and mitochondrial integrity.
- Transcriptome analysis to identify molecular pathways affected by diabetes and BGP-15 treatment.
- Evaluation of DNA damage and mitochondrial fatty acid β-oxidation.
Main Results:
- BGP-15 supplementation significantly enhanced the maturation rate of oocytes from diabetic mice.
- BGP-15 alleviated oxidative stress and mitochondrial damage in diabetic oocytes.
- Transcriptome analysis revealed diabetes-induced dysregulation in metabolic and mitochondrial pathways, which were ameliorated by BGP-15.
- BGP-15 reduced DNA damage and increased mitochondrial fatty acid β-oxidation in diabetic oocytes.
Conclusions:
- BGP-15 supplementation protects oocytes from maternal diabetes-related damage.
- BGP-15 improves oocyte quality by mitigating oxidative stress, mitochondrial dysfunction, and DNA damage.
- BGP-15 represents a promising therapeutic strategy for improving the reproductive outcomes of women with diabetes.
