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BGP-15 Mitigates Oxidative Stress and Mitochondrial Dysfunction in In Vitro Matured Oocytes From Type 1 Diabetic Mice
Luyao Wang1, Lin Yin1, Yangyang Li1
1Department of Obstetrics and Gynecology, Center for Reproductive Medicine, the Fourth Affiliated Hospital of School of Medicine, and International School of Medicine, International Institutes of Medicine, Zhejiang University, Yiwu, China.
Abstract:
Diabetes is highly associated with the decline of oocyte quality, which could further result in damage to embryo development and pregnancy outcomes. However, effective strategies for improving oocyte quality of patients with diabetes have not been fully determined. In this study, we report that supplementing BGP-15 in in vitro maturation (IVM) medium could significantly improve the maturation rate of oocytes from diabetic mice. BGP-15 supplementation could also alleviate oxidative stress and mitochondrial damage of in vitro matured oocytes from diabetic mice. Transcriptome analysis indicates that the effect of diabetes on oocytes is mainly related to dysregulation in metabolism pathways and mitochondrial-related pathways. The beneficial effect of BGP-15 on diabetic oocytes is mediated by reducing DNA damage to oocytes and increasing mitochondrial fatty acid β-oxidation. Collectively, our findings demonstrate that BGP-15 supplementation can protect oocytes from maternal diabetes-related damage and provide a potential strategy to improve reproductive outcomes of women with diabetes.
Insights
Supplementing with BGP-15 in vitro maturation (IVM) medium improves oocyte quality in diabetic mice by reducing oxidative stress and mitochondrial damage. This finding offers a potential strategy to enhance reproductive outcomes for women with diabetes.
Area of Science:
- Reproductive Biology
- Endocrinology
- Mitochondrial Medicine
Background:
- Diabetes mellitus negatively impacts oocyte quality, leading to impaired embryo development and pregnancy outcomes.
- Effective strategies to improve oocyte quality in diabetic individuals remain largely undetermined.
- Maternal diabetes poses a significant risk to reproductive health and fertility.
Purpose of the Study:
- To investigate the potential of BGP-15 as a therapeutic agent to improve oocyte quality in a mouse model of diabetes.
- To elucidate the mechanisms by which BGP-15 exerts its protective effects on diabetic oocytes.
- To assess the impact of BGP-15 on oocyte maturation rates and cellular health.
Main Methods:
- In vitro maturation (IVM) of oocytes from diabetic and control mice with and without BGP-15 supplementation.
- Assessment of oocyte maturation rates, oxidative stress markers, and mitochondrial integrity.
- Transcriptome analysis to identify molecular pathways affected by diabetes and BGP-15 treatment.
- Evaluation of DNA damage and mitochondrial fatty acid β-oxidation.
Main Results:
- BGP-15 supplementation significantly enhanced the maturation rate of oocytes from diabetic mice.
- BGP-15 alleviated oxidative stress and mitochondrial damage in diabetic oocytes.
- Transcriptome analysis revealed diabetes-induced dysregulation in metabolic and mitochondrial pathways, which were ameliorated by BGP-15.
- BGP-15 reduced DNA damage and increased mitochondrial fatty acid β-oxidation in diabetic oocytes.
Conclusions:
- BGP-15 supplementation protects oocytes from maternal diabetes-related damage.
- BGP-15 improves oocyte quality by mitigating oxidative stress, mitochondrial dysfunction, and DNA damage.
- BGP-15 represents a promising therapeutic strategy for improving the reproductive outcomes of women with diabetes.
