Lipase Production by Pseudomonas aeruginosa ATCC 9027: Process Optimization, Kinetic Modeling and Cost Assessment

Area of Science:

• Biotechnology
• Enzyme Engineering
• Microbial Fermentation

Background:

• Microbial lipases are industrially significant biocatalysts.
• Limited studies integrate media optimization, kinetic modeling, bioreactor validation, and downstream processing for Pseudomonas aeruginosa ATCC 9027 lipase production.

Purpose of the Study:

• To evaluate the lipase production potential of P. aeruginosa ATCC 9027.
• To optimize lipase production through media composition and kinetic analysis.
• To validate production in a bioreactor and assess downstream processing and costs.

Main Methods:

• Response Surface Methodology (RSM) for media optimization (olive oil, dextrose).
• Unstructured kinetic modeling for biomass growth and substrate utilization.
• Batch fermentation in a 1.5 L stirred-tank bioreactor.
• Partial purification and cost assessment.

Main Results:

• Optimized conditions achieved maximum lipase activity of 0.48 U/mL.
• Biomass growth followed the Logistic model (µmax = 0.19 h⁻¹).
• Substrate consumption indicated growth-associated utilization.
• Partial purification increased purity 2.6-fold (specific activity 223.10 U/mg).
• Bioreactor studies confirmed model predictability.

Conclusions:

• P. aeruginosa ATCC 9027 is a promising microbial lipase producer.
• Integration of kinetic understanding, bioreactor validation, and downstream processing is crucial for scale-up.
• The study provides a framework for techno-economic evaluation of lipase production.