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Updated: Apr 12, 2026
An Effective Mouse Model of Unilateral Renal Ischemia-Reperfusion Injury
Published on: July 15, 2021
Multiparametric MRI as predictors for evaluating renal ischemia reperfusion injury and protective effect of
Jin-Lu Song1, Zhen Xu1, Yu-Hong Wang2
1Medical Imaging Center, The First Affiliated Hospital of Jinan University, No. 613 West Huangpu Avenue, Tianhe District, Guangzhou, Guangdong, 510630, China.
Background:
We aimed to evaluate multiparametric magnetic resonance imaging (MRI), including blood oxygen level dependent (BOLD), arterial spin labeling (ASL), and intravoxel incoherent motion (IVIM) for noninvasively monitoring acute kidney injury (AKI) to acute kidney disease (AKD) progression following renal ischemia reperfusion injury (IRI) and the subsequent response to Mito-TEMPO (MT) therapy.
Methods:
Phase 1: 42 Sprague Dawley (SD) rats (36 IRI and 6 control). Phase 2: 144 SD rats (IRI/control + MT treatment/saline, 36 per group). IRI group: left renal ischemia reperfusion procedures, control group: sham abdominal surgery. Animals underwent MRI scanning to obtain effective transverse relaxation rate (R2*), renal blood flow (RBF), apparent diffusion coefficient (ADC), pure molecular diffusion (D), perfusion-related diffusion (D*), and perfusion fraction (f) values of kidney. After sacrifice, serological and histological analysis were performed.
Results:
All MRI parameters (R2*, RBF, ADC, D*, D, f) showed significant, time-dependent alterations following IRI compared to controls. These changes were effectively reversed by MT treatment, with most parameters recovering to near-baseline levels.
Conclusion:
BOLD, ASL, and IVIM can be used as noninvasive radiologic markers for detecting the progression from renal IRI to AKI-AKD and recovery after MT treatment.
Insights
Multiparametric MRI, including BOLD, ASL, and IVIM, can noninvasively detect acute kidney injury (AKI) progression after renal ischemia reperfusion injury (IRI). These imaging markers also monitor recovery following Mito-TEMPO (MT) therapy.
Area of Science:
- Nephrology
- Radiology
- Biomedical Engineering
Background:
- Acute kidney injury (AKI) and acute kidney disease (AKD) following renal ischemia reperfusion injury (IRI) require effective monitoring methods.
- Multiparametric MRI techniques, including blood oxygen level dependent (BOLD), arterial spin labeling (ASL), and intravoxel incoherent motion (IVIM), offer potential for noninvasive assessment.
- Mito-TEMPO (MT) therapy is being investigated for its therapeutic effects in IRI-induced AKI.
Purpose of the Study:
- To evaluate the utility of multiparametric MRI (BOLD, ASL, IVIM) for noninvasively monitoring the progression of AKI to AKD after renal IRI.
- To assess the ability of these MRI parameters to track the response to MT therapy in a rat model of renal IRI.
Main Methods:
- The study involved two phases with Sprague Dawley rats, including IRI and sham surgery groups.
- Animals underwent multiparametric MRI to obtain R2*, renal blood flow (RBF), apparent diffusion coefficient (ADC), pure molecular diffusion (D), perfusion-related diffusion (D*), and perfusion fraction (f) values.
- Post-sacrifice, serological and histological analyses were performed for correlation.
Main Results:
- All evaluated MRI parameters (R2*, RBF, ADC, D*, D, f) demonstrated significant, time-dependent changes post-IRI compared to controls.
- MT treatment effectively reversed these IRI-induced alterations, with most MRI parameters returning to near-baseline levels.
- These findings suggest a strong correlation between MRI metrics and kidney injury/recovery status.
Conclusions:
- Multiparametric MRI (BOLD, ASL, IVIM) serves as a reliable noninvasive tool for detecting renal IRI progression to AKI-AKD.
- These imaging modalities can effectively monitor the recovery process following MT treatment.
- The study highlights the potential of advanced MRI techniques in managing kidney injury.
