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Cell death assays for the detection of acute kidney tubular necrosis

Francesca Maremonti1, Andreas Linkermann2

  • 1Department of Medicine V, University Medical Centre Mannheim, University of Heidelberg, Mannheim, Germany; Division of Nephrology, Department of Internal Medicine 3, University Hospital Carl Gustav Carus at the Technische Universität Dresden, Dresden, Germany.

Methods in Cell Biology
|April 11, 2026
PubMed

Insights

Investigating acute tubular necrosis (ATN) requires reliable methods to study cell death. This work details techniques for isolating kidney tubules and assays to detect regulated necrosis, aiding research into kidney failure.

Area of Science:

  • Nephrology
  • Cell Biology
  • Molecular Biology

Background:

  • Acute tubular necrosis (ATN) is a primary cause of nephron loss and kidney failure.
  • Understanding the cellular mechanisms of tubular necrosis is crucial for developing treatments.
  • Reliable models are needed to quantify cell death in kidney tubules.

Purpose of the Study:

  • To introduce methodologies for isolating murine renal tubules ex vivo.
  • To describe assays for detecting regulated necrosis in kidney tubules.
  • To provide a framework for studying tubular cell death pathways.

Main Methods:

  • Isolation of murine renal tubules ex vivo.
  • Biochemical assays for cell death detection.
  • Molecular assays for necrosis pathway analysis.
  • Imaging approaches for visualizing tubular injury.

Main Results:

  • Established protocols for ex vivo renal tubule isolation.
  • Validated a panel of assays to quantify regulated necrosis.
  • Demonstrated the utility of these methods in studying kidney tubule cell death.

Conclusions:

  • The described methodologies provide a robust system for studying acute tubular necrosis.
  • These techniques facilitate the dissection of cellular pathways involved in nephron loss.
  • This approach aids in the development of therapeutic strategies for kidney failure.
Keywords:
Acute kidney injuryAcute tubular necrosisFerroptosisKidney tubulesLDHLive-cell imagingPrimary tubular cellsRegulated necrosisWestern blotex vivo

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