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Related Experiment Video

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Detecting phosphorylated MLKL in murine tissues.

Riley M Williams1, Bikash Thapa1, Siddharth Balachandran1

  • 1Center for Immunology, Fox Chase Cancer Center, Philadelphia, PA, United States.

Methods in Cell Biology
|April 11, 2026
PubMed
Summary
This summary is machine-generated.

Necroptosis, a programmed cell death, is triggered by RIPK3 phosphorylating MLKL. This study details detecting phosphorylated MLKL in influenza A virus-infected lungs and tumors treated with CBL0137.

Keywords:
CBL0137Influenza A virusMLKLNecroptosisRIPK3Z-DNAZ-RNAZBP1

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Area of Science:

  • Biochemistry
  • Cell Biology
  • Immunology

Background:

  • Necroptosis is a programmed form of necrosis.
  • Receptor Interacting protein Kinase 3 (RIPK3) phosphorylates Mixed Lineage Kinase Like protein (MLKL) to activate necroptosis.
  • Phosphorylated MLKL forms pores in cellular membranes, leading to cell death.

Purpose of the Study:

  • To describe a procedure for detecting phosphorylated MLKL.
  • To assess phosphorylated MLKL in influenza A virus (IAV)-infected lung tissues.
  • To evaluate phosphorylated MLKL in tumors treated with CBL0137.

Main Methods:

  • Detection of phosphorylated murine MLKL.
  • Analysis of lung tissues from IAV-infected mice.
  • Analysis of tumor tissues from mice treated with CBL0137.

Main Results:

  • The described procedure allows for the detection of phosphorylated MLKL.
  • Phosphorylated MLKL was detected in IAV-infected lung tissues.
  • Phosphorylated MLKL was detected in tumors treated with CBL0137.

Conclusions:

  • Phosphorylation of MLKL by RIPK3 is a key event in necroptosis.
  • Detecting phosphorylated MLKL can serve as a biomarker for necroptosis.
  • This method can be applied to study necroptosis in various disease models.