Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Studying the Cytoskeleton01:17

Studying the Cytoskeleton

10.5K
The cytoskeletal architecture can be studied using different microscopic and biochemical techniques. Electron microscopy was instrumental in discovering the cytoskeletal architecture around the 1960s, which allowed obtaining structural information at a high-resolution level. However, the sample preparation procedure often limits this ability in biological samples. Several protocols have been developed over the years to optimize sample preparation. In one of the protocols known as rotary...
10.5K
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

2.8K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

DNAM-1 mediates NK-cell activation and host-pathogen interaction via direct binding to fungal cell wall proteases.

Communications biology·2026
Same author

Loss of GPRC5D enhances the proliferative capacity and competitive fitness of myeloma upon anti-GPRC5D immunotherapy.

Leukemia·2026
Same author

Impact of Docking Strand Design on Spatial Resolution in DNA-Points Accumulation for Imaging in Nanoscale Topography.

Chemphyschem : a European journal of chemical physics and physical chemistry·2026
Same author

Single-molecule localization microscopy reveals the molecular organization of endogenous membrane receptors.

Science advances·2026
Same author

Platelet-derived integrin- and tetraspanin-enriched tethers exacerbate severe inflammation.

Science (New York, N.Y.)·2026
Same author

A reliable in vitro rumen culture system and workflow for screening anti-methanogenic compounds.

PloS one·2025
Same journal

Synergistic Visible-Light-Driven CO<sub>2</sub> Reduction and H<sub>2</sub>O Oxidation over Ti<sub>3</sub>C<sub>2</sub> Quantum Dot-Modified Cu/g-C<sub>3</sub>N<sub>4</sub> Photocatalysts.

Langmuir : the ACS journal of surfaces and colloids·2026
Same journal

Spontaneous Phase Separation Enables Rapid, Polymerization-Free Fabrication of Gels.

Langmuir : the ACS journal of surfaces and colloids·2026
Same journal

Lamellar-Confinement-Induced ZIF-67 Nanosheet Mixed Matrix Membranes for Enhanced CH<sub>4</sub>/N<sub>2</sub> Separation.

Langmuir : the ACS journal of surfaces and colloids·2026
Same journal

Structure Control of Oblate Nanoparticles Self-Assembled by ABC Cyclic Terpolymers under Soft Confinement.

Langmuir : the ACS journal of surfaces and colloids·2026
Same journal

Tuning Brønsted/Lewis Acid Site Ratios via Ammonia Modulation for Selective Conversion of Glycerol to 1,3-Propanediol or Solketal.

Langmuir : the ACS journal of surfaces and colloids·2026
Same journal

Catalytic and Nitriding Competition of Nitrogen Atom on Graphene and Its Finite Rate Surface Chemistry Model.

Langmuir : the ACS journal of surfaces and colloids·2026
See all related articles

Related Experiment Video

Updated: Apr 13, 2026

Directly Measuring Forces Within Reconstituted Active Microtubule Bundles
07:47

Directly Measuring Forces Within Reconstituted Active Microtubule Bundles

Published on: May 10, 2022

2.0K

Traction Force Microscopy with DNA FluoroCubes.

Armina Mortazavi1,2,3, Jianfei Jiang1,2,3, Philip Laric2

  • 1Department of Physics, Technische Universität Dortmund, Otto-Hahn-Str. 4, Dortmund 44221, Germany.

Langmuir : the ACS Journal of Surfaces and Colloids
|April 12, 2026
PubMed
Summary
This summary is machine-generated.

Researchers developed FluoroCubes, DNA nanostructures for high-resolution cell force mapping. This new method improves traction force microscopy (TFM) by providing stable, dense fiducial markers on substrates.

More Related Videos

Author Spotlight: Efficient Nucleosome Reconstitution for Single-Molecule Techniques
05:58

Author Spotlight: Efficient Nucleosome Reconstitution for Single-Molecule Techniques

Published on: September 6, 2024

1.8K
High-Speed Atomic Force Microscopy Imaging of DNA Three-Point-Star Motif Self Assembly Using Photothermal Off-Resonance Tapping
08:59

High-Speed Atomic Force Microscopy Imaging of DNA Three-Point-Star Motif Self Assembly Using Photothermal Off-Resonance Tapping

Published on: March 22, 2024

1.3K

Related Experiment Videos

Last Updated: Apr 13, 2026

Directly Measuring Forces Within Reconstituted Active Microtubule Bundles
07:47

Directly Measuring Forces Within Reconstituted Active Microtubule Bundles

Published on: May 10, 2022

2.0K
Author Spotlight: Efficient Nucleosome Reconstitution for Single-Molecule Techniques
05:58

Author Spotlight: Efficient Nucleosome Reconstitution for Single-Molecule Techniques

Published on: September 6, 2024

1.8K
High-Speed Atomic Force Microscopy Imaging of DNA Three-Point-Star Motif Self Assembly Using Photothermal Off-Resonance Tapping
08:59

High-Speed Atomic Force Microscopy Imaging of DNA Three-Point-Star Motif Self Assembly Using Photothermal Off-Resonance Tapping

Published on: March 22, 2024

1.3K

Area of Science:

  • Biophysics
  • Cell Biology
  • Materials Science

Background:

  • Mechanical forces at the cell-substrate interface regulate crucial cellular processes.
  • High-resolution mapping of these forces is essential but technically challenging.
  • Conventional traction force microscopy (TFM) uses fluorescent beads as fiducial markers.

Purpose of the Study:

  • To introduce and validate fluorescently labeled DNA nanostructures (FluoroCubes) as advanced fiducial markers for TFM.
  • To enhance the spatial resolution and sensitivity of traction force measurements.
  • To develop a versatile platform for probing interfacial forces at biointerfaces.

Main Methods:

  • Grafting DNA nanostructures (FluoroCubes) onto polydimethylsiloxane (PDMS) substrates, co-anchored with RGD peptides.
  • Utilizing biotin-NeutrAvidin chemistry for controlled fiducial density.
  • Employing total internal reflection fluorescence (TIRF) microscopy.
  • Applying a modified multichannel optical flow algorithm for displacement and force reconstruction.

Main Results:

  • FluoroCubes offer stable tethering, resist internalization, and enable dense, minimally perturbative labeling.
  • The platform demonstrates improved displacement sensitivity and force reconstruction resolution compared to conventional methods.
  • Achieved reproducible, high-resolution traction force mapping.

Conclusions:

  • FluoroCube-functionalized substrates represent a significant advancement in traction force microscopy.
  • This method provides a reproducible and high-resolution approach for mapping interfacial mechanical forces.
  • The platform is versatile for future integration with DNA-based molecular sensors for advanced biointerface studies.