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Genome-wide identification and functional validation of thermal tolerance genes in Mytilus coruscus using a
Chao Li1, Heng Zhou1, Siying Lv2
1National Engineering Research Center of Marine Facilities Aquaculture, Zhejiang Ocean University, Zhoushan, 316022, China.
International Journal of Biological Macromolecules
|April 12, 2026
Summary
Marine mussels like Mytilus coruscus face heat stress from ocean warming. This study identified key heat shock proteins and genes crucial for their thermotolerance, offering insights for breeding resilient shellfish.
Area of Science:
- Marine Biology
- Molecular Biology
- Genomics
Background:
- Ocean warming poses a significant threat to marine bivalves, impacting their physiology and heat tolerance.
- Mytilus coruscus is particularly vulnerable to rising temperatures, yet its molecular thermotolerance mechanisms remain largely unknown.
Purpose of the Study:
- To investigate the molecular mechanisms underlying thermotolerance in Mytilus coruscus under heat stress.
- To identify genes and pathways involved in oxidative stress response and heat adaptation.
Main Methods:
- Exposure of mussels to graded heat stress to analyze oxidative responses and gene expression.
- Construction and functional screening of a heat-stress cDNA library using yeast heterologous expression.
- Integration of transcriptomic data with functional screening results.
- Protein-protein interaction analysis.
Main Results:
- Heat stress induced oxidative stress genes and activated antioxidant and heat shock protein pathways in a temperature-dependent manner.
- Identified 716 candidate thermotolerance genes, with 235 core upregulated genes enriched in heat response and protein stability.
- Core genes included heat shock proteins (HSP70, HSP90, sHSPs); downregulated genes were linked to stem cell division and regeneration.
- Identified potential regulatory interactions between heat shock transcription factor and core thermotolerance proteins.
Conclusions:
- Mytilus coruscus exhibits a complex thermotolerance response involving oxidative stress management and heat shock protein activation.
- The study provides a comprehensive set of core thermotolerance genes for M. coruscus.
- Findings offer valuable genetic resources for developing heat-resilient bivalve aquaculture and conservation strategies.
