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Related Concept Videos

CRISPR01:59

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR/Cas9 Genome Editing01:28

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
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Rapid identification of binary and ternary adulteration in camellia oil by CRISPR/Cas12a assay.

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Related Experiment Video

Updated: Apr 14, 2026

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases
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A universal light-controlled highly sensitive one-pot CRISPR/Cas12a diagnostic based on structure-engineered crRNA.

Jieyu Cui1, Lulu Zhang2, Jing Zhou2

  • 1State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, and College of Veterinary Medicine, Jilin University, Changchun 130062, China; State Key Laboratory of Genome and Multi-omics Technologies, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China.

Trends in Biotechnology
|April 12, 2026
PubMed
Summary
This summary is machine-generated.

A new CRISPR/Cas12a testing platform (ULTRAt) enhances one-pot nucleic acid detection sensitivity. This light-controlled method improves speed and accuracy for near-patient diagnostics.

Keywords:
CRISPR/Cas12alight controlone-pot detectionpoint-of-care diagnosticsscaffold

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Area of Science:

  • Molecular diagnostics
  • Biotechnology
  • CRISPR-based detection

Background:

  • CRISPR-based nucleic acid detection offers speed and accuracy.
  • One-pot formats face limitations in sensitivity and field suitability.

Purpose of the Study:

  • To develop a universal light-controlled, high-sensitivity, one-pot CRISPR/Cas12a testing (ULTRAt) platform.
  • To overcome sensitivity and enrichment limitations in existing one-pot CRISPR assays.

Main Methods:

  • Developed structure-engineered CRISPR RNA (crRNA) scaffolds with photocaged groups.
  • Utilized UV irradiation to control Cas12a activity and enable target enrichment.
  • Integrated isothermal amplification with CRISPR/Cas12a trans-cleavage.

Main Results:

  • Achieved a limit of detection of two copies of monkeypox virus per reaction.
  • Demonstrated a 100-fold sensitivity improvement over conventional assays with a 15-min time-to-result.
  • Successfully performed single-nucleotide polymorphism discrimination and HPV genotyping, with strong clinical sample concordance.

Conclusions:

  • The ULTRAt platform enables rapid, ultra-sensitive, and versatile one-pot detection.
  • ULTRAt supports near-patient diagnostics and genotyping applications.
  • This technology advances molecular diagnostics with improved sensitivity and field suitability.