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Updated: Apr 14, 2026

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CRISPR-mediated Genome Editing of the Human Fungal Pathogen Candida albicans
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Marker-Free CRISPR/Cas9 RNP Transformation for Gene Disruption in Aspergillus fumigatus
Clara Valero1, Jamie Tindale1, Michael J Bromley2
1Manchester Fungal Infection Group, University of Manchester, Manchester, UK.
Methods in Molecular Biology (Clifton, N.J.)
|April 12, 2026
Abstract:
CRISPR-based genetic engineering has resulted in the possibility of far more efficient and challenging genetic engineering in filamentous fungal pathogens. In this respect, selection marker-free CRISPR-Cas9 transformation has been possible in fungi such as Aspergillus fumigatus, one of the most prevalent fungal pathogens of humans. Here, we describe a protocol to perform this technique, which can be widely adapted to use multiple strains and other species.

