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Updated: Apr 14, 2026
Bronchoalveolar Lavage of Murine Lungs to Analyze Inflammatory Cell Infiltration
Published on: May 4, 2017
Bronchoalveolar Lavage Fluid Collection in Invasive Pulmonary Aspergillosis Murine Model for Downstream Proteomic
1Institut Pasteur, Université de Paris, Immunology of Fungal Infections, Paris, France. sarah.wong@pasteur.fr.
Abstract:
The immunocompromised populations are susceptible to invasive fungal infections, such as invasive pulmonary aspergillosis (IPA). Aspergillus spp. produce airborne spores, called conidia. The small size of conidia allows them to reach the most proximal airways, such as the alveoli. The bronchoalveolar lavage fluid represents an important source of proteins, either from the host or the fungal pathogen, that are associated with the pathobiology of Aspergillus fumigatus and, thus, serve as crucial factors to elucidate virulence factors and/or promising novel antifungal drug targets. This chapter describes the method to obtain bronchoalveolar lavage fluid from an immunosuppressed murine model of IPA and the protein concentration estimation required for downstream proteomic analysis of the bronchoalveolar lavage fluid.
Insights
Immunocompromised individuals are prone to invasive pulmonary aspergillosis (IPA). This study details methods for collecting bronchoalveolar lavage fluid from mice to analyze proteins for new antifungal drug targets.
Area of Science:
- Medical Mycology
- Proteomics
- Infectious Diseases
Background:
- Invasive pulmonary aspergillosis (IPA) poses a significant threat to immunocompromised populations.
- Aspergillus fumigatus conidia can reach deep into the lungs, causing infection.
- Bronchoalveolar lavage (BAL) fluid contains host and fungal proteins crucial for understanding IPA pathogenesis.
Purpose of the Study:
- To describe the methodology for obtaining BAL fluid from an immunosuppressed murine model of IPA.
- To outline protein concentration estimation techniques for BAL fluid.
- To facilitate downstream proteomic analysis for identifying virulence factors and drug targets.
Main Methods:
- Establishment of an immunosuppressed murine model.
- Collection of bronchoalveolar lavage fluid.
- Protein concentration determination using established assays.
Main Results:
- Successful isolation of BAL fluid from the murine model.
- Quantification of protein content in BAL fluid samples.
- Preparation of samples for proteomic analysis.
Conclusions:
- The described methods enable the collection and analysis of BAL fluid in an IPA mouse model.
- This approach is vital for identifying Aspergillus fumigatus virulence factors.
- The findings support the development of novel antifungal therapies.
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