SlNAP1-SlGAI-SlAOS1 module regulates jasmonic acid biosynthesis mediating the low-temperature stress response in...

Area of Science:

• Plant Biology
• Molecular Genetics
• Stress Physiology

Background:

• Abiotic stresses, like low temperatures, significantly impact crop yield and quality globally.
• Transcription factor (TF) families, such as NAM, ATAF, and CUC (NAC), are crucial regulators of plant responses to environmental stress.

Purpose of the Study:

• To investigate the role of the NAC TF family member SlNAP1 in tomato's response to low-temperature stress.
• To elucidate the molecular mechanism involving SlNAP1, SlGAI, and SlAOS1 in jasmonic acid (JA) biosynthesis and low-temperature tolerance.

Main Methods:

• Gene expression analysis of SlNAP1 under low-temperature and jasmonic acid (JA) treatments.
• Investigating the regulatory relationship between SlNAP1 and SlAOS1 using molecular biology techniques.
• Analyzing the phenotypic effects of SlAOS1 overexpression and knockout under low-temperature stress.
• Identifying and characterizing the interaction between SlGAI and SlNAP1.

Main Results:

• SlNAP1 expression is significantly induced by low-temperature and JA signals.
• SlNAP1 directly targets and regulates SlAOS1, promoting JA biosynthesis and enhancing low-temperature tolerance in tomatoes.
• Overexpression of SlAOS1 increases JA accumulation and reduces reactive oxygen species (ROS) under low-temperature stress.
• SlGAI interacts with SlNAP1, enhancing its regulatory function on SlAOS1, leading to increased JA and reduced ROS.

Conclusions:

• The SlNAP1-SlGAI-SlAOS1 module is a key regulator of JA biosynthesis in tomatoes.
• This module plays a vital role in mediating tomato's response to low-temperature stress by modulating JA accumulation and ROS levels.