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Related Experiment Video

Updated: Apr 14, 2026

High-Resolution Endocardial and Epicardial Optical Mapping in a Sheep Model of Stretch-Induced Atrial Fibrillation

High-Resolution Endocardial and Epicardial Optical Mapping in a Sheep Model of Stretch-Induced Atrial Fibrillation

Published on: July 29, 2011

From Pig to Human: Endo-Epicardial Substrate Characterization Using Dual Optical Mapping.

Jimena Siles^1,2, Casey Lee-Trimble², Evan Rheaume²

¹HEartLab, Federal University of ABC, São Bernardo do Campo, Brazil.

Computing in Cardiology

|April 13, 2026

Summary

This summary is machine-generated.

Dual optical mapping reveals critical differences in how porcine and human hearts develop arrhythmias. Human hearts show more alternans and heterogeneity, highlighting species-specific mechanisms for sudden cardiac arrest.

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Optocardiography and Electrophysiology Studies of Ex Vivo Langendorff-perfused Hearts

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Optocardiography and Electrophysiology Studies of Ex Vivo Langendorff-perfused Hearts

Optocardiography and Electrophysiology Studies of Ex Vivo Langendorff-perfused Hearts

Published on: November 7, 2019

Area of Science:

Cardiovascular Physiology
Cardiac Electrophysiology
Arrhythmogenesis Research

Background:

Understanding endocardial and epicardial electrical activity dissociation is key to investigating arrhythmia mechanisms.
Dual-surface mapping provides insights into the complex electrical behavior of the heart.

Purpose of the Study:

To simultaneously record transmembrane voltage from endocardial and epicardial surfaces in porcine and human hearts.
To investigate species-specific differences in arrhythmogenesis using dual optical mapping.

Main Methods:

Utilized a dual optical mapping system for simultaneous endo-epi recording.
Paced endocardium at increasing rates (PCL 1000 ms to block/arrhythmia).
Analyzed action potential duration (APD) alternans and repolarization heterogeneity.

Main Results:

Porcine hearts transitioned abruptly to fibrillation with minimal APD alternans.
Human hearts displayed significant APD alternans and repolarization heterogeneity before fibrillation.
Endocardial maps during fibrillation showed conduction block and reentrant sites; epicardial activation was slower.

Conclusions:

Demonstrated endo-epicardial dissociation and species-specific differences in arrhythmogenesis.
Dual-surface optical mapping is a valuable tool for translational cardiac research.
Findings advance understanding of arrhythmia mechanisms and potential therapeutic targets.