Pseudorabies virus infection reshapes the host epitranscriptome by globally suppressing but selectively elevating...

Area of Science:

• * Molecular Biology
• * Virology
• * Epigenetics

Background:

• * RNA modifications are vital for cellular processes and increasingly recognized in viral infections.
• * While N6-methyladenosine (m6A) is studied, other RNA modifications during viral infections are less understood.
• * Pseudorabies virus (PRV) is a significant pathogen affecting swine health.

Purpose of the Study:

• * To comprehensively map RNA modification profiles in porcine kidney PK-15 cells during PRV infection.
• * To identify changes in specific RNA modifications and associated enzymes in response to PRV.

Main Methods:

• * Analysis of RNA modification landscapes in PRV-infected porcine kidney PK-15 cells.
• * Quantification of 31 distinct RNA modifications across different infection time points.
• * Investigation of enzyme levels related to m1A modification.

Main Results:

• * PRV infection altered the abundance of 31 distinct RNA modifications, with 25 showing increased levels.
• * Overall RNA modification levels were suppressed, but specific modifications (Am, Cm, Gm, Um) were elevated.
• * PRV infection significantly reduced the levels of enzymes involved in m1A modification.

Conclusions:

• * PRV infection profoundly impacts the host cell's RNA modification landscape.
• * The study reveals specific RNA modifications that are modulated during PRV infection.
• * Findings provide a foundation for understanding RNA modifications in herpesvirus infections and potential therapeutic targets.