Genome-Wide Identification of MYB Genes and Analysis of Their Expression Under Cold Stress Conditions in Artocarpus...

Jackfruit (Artocarpus heterophyllus) thrives in subtropical and tropical areas but is vulnerable to winter cold stress. The myeloblastosis (MYB) transcription factor family plays an important role in plant biological and abiotic stress responses; however, their response to regional cold injury in jackfruit remains unexplored. The study aimed to identify and analyze the expression of 298 MYB genes in jackfruit under cold stress conditions. The study used genome-wide identification, bioinformatics analysis, and qPCR to analyze the expression of 298 MYB genes in jackfruit under cold stress. Evolutionary tree analysis showed that the AhMYB family members were divided into seven subfamilies. Chromosome mapping results showed that 298 AhMYB family members were unevenly distributed on 27 chromosomes. In addition, analysis of conserved motifs and gene structure showed that members of the AhMYB family located in the same subfamily had similar conserved motifs and gene structure. Collinear analysis identified 1439 duplicated fragments involving AhMYB family members within the species. In addition, the cis-acting elements in the promoters of AhMYB family members are implicated in many aspects of plant growth and development. Transcriptomic analysis of jackfruit under low-temperature stress showed that the expression patterns of AhMYB family members differed in jackfruit varieties. qPCR analysis further verified this result, confirming that AhMYB family members are involved in the response to low-temperature stress in jackfruit varieties. These findings provide new insights into the functions of AhMYB family members.