Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Microbial Biosensors01:17

Microbial Biosensors

62
Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...
62

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Determination and risk assessment of ptaquiloside from bracken fern in Korea using monitoring data.

Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment·2026
Same author

Intravenous MR Lymphangiography: Feasibility in a Swine Model and Proof-of-Concept in Patients.

Radiology·2026
Same author

Oncolytic adenovirus co-expressing IL-12 and shVEGF potentiates immune checkpoint blockade for treatment of renal cell carcinoma: in vitro, organ-on-a-chip, and in vivo evaluation.

Molecular medicine (Cambridge, Mass.)·2026
Same author

Efficacy of oral alitretinoin for concomitant foot eczema in chronic hand eczema.

Journal of the European Academy of Dermatology and Venereology : JEADV·2026
Same author

Effects of a practitioner-led empowerment program for low-income social service recipients in South Korea: a quasi-experimental study.

Journal of Korean Academy of Nursing·2026
Same author

Feasibility of a short-term intensive home-based cognitive and physical training program for older adults: a single-arm pilot study.

BMC geriatrics·2026

Related Experiment Video

Updated: Apr 14, 2026

Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance SPR
09:35

Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance SPR

Published on: November 29, 2014

23.8K

Nanogel-Modified SPR Sensors Enable Specific Biomarker Detection in Complex Media through Minimum Response Analysis.

Suhwan Jeon1, Jie Ying Teoh1, Eunyoung Park1

  • 1Department of Chemical and Biological Engineering, and Institute of Chemical Processes, Seoul National University, Seoul 08826, Republic of Korea.

ACS Sensors
|April 13, 2026
PubMed
Summary

This study introduces a novel nanogel-modified surface plasmon resonance (SPR) interface for direct, label-free detection of interleukin-6 (IL-6) in human serum. The new method enhances sensitivity and diagnostic accuracy in complex biological samples.

Keywords:
biomarker detectionhuman serum analysismultivalent protein bindingnanogelsurface plasmon resonance

More Related Videos

Using Extraordinary Optical Transmission to Quantify Cardiac Biomarkers in Human Serum
09:23

Using Extraordinary Optical Transmission to Quantify Cardiac Biomarkers in Human Serum

Published on: December 13, 2017

6.8K
Electronic Tongue Generating Continuous Recognition Patterns for Protein Analysis
08:46

Electronic Tongue Generating Continuous Recognition Patterns for Protein Analysis

Published on: September 16, 2014

8.3K

Related Experiment Videos

Last Updated: Apr 14, 2026

Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance SPR
09:35

Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance SPR

Published on: November 29, 2014

23.8K
Using Extraordinary Optical Transmission to Quantify Cardiac Biomarkers in Human Serum
09:23

Using Extraordinary Optical Transmission to Quantify Cardiac Biomarkers in Human Serum

Published on: December 13, 2017

6.8K
Electronic Tongue Generating Continuous Recognition Patterns for Protein Analysis
08:46

Electronic Tongue Generating Continuous Recognition Patterns for Protein Analysis

Published on: September 16, 2014

8.3K

Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Biotechnology

Background:

  • Direct, label-free biomarker quantification in human serum using Surface Plasmon Resonance (SPR) is hindered by nonspecific adsorption and matrix effects.
  • Developing robust SPR interfaces for complex biological media is crucial for sensitive and reliable biomarker detection.

Purpose of the Study:

  • To develop a novel nanogel-modified SPR interface for direct, label-free quantification of interleukin-6 (IL-6) in human serum.
  • To leverage multivalent protein binding (MPB) for signal amplification and enhance detection sensitivity and stability.
  • To introduce and validate a new analytical parameter, minimum response (Rmin), for improved diagnostic performance in complex samples.

Main Methods:

  • Fabrication of a novel NTA-conjugated pNIPAM-co-AAc nanogel-modified SPR interface (NTA-NG).
  • Immobilization of His-tagged GP130 on the nanogel surface to form an IL-6-IL-6R-mediated ternary complex for MPB-driven signal transduction.
  • Comparison of NTA-NG platform performance against planar NTA-COOH surfaces and dextran-based CM5 interfaces.
  • Introduction and application of the minimum response (Rmin) as an analytical parameter.

Main Results:

  • The NTA-NG platform demonstrated enhanced robustness, signal stability, and sensitivity for IL-6 detection in human serum compared to conventional SPR interfaces.
  • The minimum response (Rmin) parameter showed superior signal discrimination and diagnostic performance (AUC = 0.918) in clinical serum samples compared to the maximum response (Rmax) (AUC = 0.833).
  • Rmin-based analysis results correlated well with ELISA, indicating high accuracy.

Conclusions:

  • The NTA-NG modified SPR interface provides a reliable and sensitive platform for direct, label-free IL-6 quantification in complex biological matrices like human serum.
  • The minimum response (Rmin) parameter offers improved analytical robustness and diagnostic utility for biomarker detection in challenging clinical samples.
  • This approach holds significant potential for advancing diagnostic tools in oncology and other fields requiring sensitive biomarker analysis.