Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Viruses with RNA Genomes

Viruses with RNA Genomes

RNA viruses are categorized into positive-strand, negative-strand, or double-stranded groups based on their genomic structure and replication mechanisms. This classification dictates how they exploit host cellular machinery for protein synthesis and replication. Some RNA viruses also utilize reverse transcription as part of their life cycle, further diversifying their replication strategies.Positive-Strand RNA VirusesPositive-strand RNA viruses have genomes that function directly as messenger...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Anti-inflammatory effects of water-soluble polysaccharides from Annona squamosa L. pulp in LPS-induced zebrafish embryos: Network pharmacology-guided analysis and experimental validation.

Journal of ethnopharmacology·2026

Same author

Author Correction: TGF-β promotes pericyte-myofibroblast transition in subretinal fibrosis through the Smad2/3 and Akt/mTOR pathways.

Experimental & molecular medicine·2026

Same author

Cuprorivaite as a Multifunctional Material for Hypertrophic Scar Modulation with Intrinsic Photothermal Enhancement.

ACS applied materials & interfaces·2026

Same author

Research advances in claudin-1 in the eye.

Experimental eye research·2026

Same author

Bidirectional associations of depression, anxiety, sleep disorders, and constipation: insights from Mendelian randomization.

Archives of medical science : AMS·2026

Same author

Lipid trapping slows ball-and-chain inactivation in a calcium-activated potassium channel.

Nature communications·2026

Search research articles

Related Experiment Video

Updated: Apr 14, 2026

Surface Functionalization of Hepatitis E Virus Nanoparticles Using Chemical Conjugation Methods

Surface Functionalization of Hepatitis E Virus Nanoparticles Using Chemical Conjugation Methods

Published on: May 11, 2018

Self-Assembling Enveloped Virus-Mimicking Particle for Extrahepatic Targeting mRNA Delivery.

Renhe Yu¹, Yukun Huang¹, Wen Shi¹

¹Department of Pharmacology and Chemical Biology, Shanghai Universities Collaborative Innovation Center for Translational Medicine, Shanghai Jiao Tong University School of Medicine, Shanghai 200120, China.

|April 13, 2026

Summary

This summary is machine-generated.

Researchers developed enveloped virus-mimicking particles (EVMPs) for efficient messenger RNA (mRNA) delivery to extrahepatic tissues. This biomimetic nanoplatform shows promise for treating lung diseases and tumors with enhanced safety and efficacy.

Keywords:

bottom up directed evolution enveloped virus-mimicking particle extrahepatic mRNA delivery self-assembling domain virus-mimicking peptide

More Related Videos

Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA

Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA

Published on: February 1, 2019

Delivery of Exogenous Artificially Synthesized miRNA Mimic to the Kidney Using Polyethylenimine Nanoparticles in Several Kidney Disease Mouse Models

Delivery of Exogenous Artificially Synthesized miRNA Mimic to the Kidney Using Polyethylenimine Nanoparticles in Several Kidney Disease Mouse Models

Published on: May 10, 2022

Related Experiment Videos

Last Updated: Apr 14, 2026

Surface Functionalization of Hepatitis E Virus Nanoparticles Using Chemical Conjugation Methods

Surface Functionalization of Hepatitis E Virus Nanoparticles Using Chemical Conjugation Methods

Published on: May 11, 2018

Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA

Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA

Published on: February 1, 2019

Delivery of Exogenous Artificially Synthesized miRNA Mimic to the Kidney Using Polyethylenimine Nanoparticles in Several Kidney Disease Mouse Models

Delivery of Exogenous Artificially Synthesized miRNA Mimic to the Kidney Using Polyethylenimine Nanoparticles in Several Kidney Disease Mouse Models

Published on: May 10, 2022

Area of Science:

Biomaterials Science
Nanotechnology
Molecular Biology

Background:

Messenger RNA (mRNA) therapeutics require efficient delivery to extrahepatic tissues, a significant challenge.
Existing viral and virus-like particle (VLP) delivery systems face limitations in safety, immunogenicity, and scalability.
A need exists for tunable, safe, and scalable delivery vehicles for extrahepatic mRNA therapy.

Purpose of the Study:

To engineer a novel, bottom-up self-assembling enveloped virus-mimicking particle (EVMP) for efficient extrahepatic mRNA delivery.
To optimize EVMP composition and structure for enhanced tissue targeting and transfection efficiency.
To evaluate the therapeutic efficacy and biosafety of EVMPs in preclinical models.

Main Methods:

Utilized virtual screening via molecular dynamics simulations for particle design.
Employed directed evolution through key assembling domain mutation for optimization.
Incorporated N-terminal fatty acylation and adjusted envelope phospholipid composition.
Tested EVMP delivery efficiency in lung and spleen tissues and in a lung tumor model.

Main Results:

Achieved high-efficiency mRNA delivery to lungs and spleen.
Optimized lung-targeted EVMPs transfected 37% of lung cells, including endothelial and immune cells.
Demonstrated significant suppression of metastatic lung tumor progression using IL-12 mRNA-loaded EVMPs.
EVMPs exhibited excellent long-term biosafety and tolerability for repeated administration due to minimal immunogenicity.

Conclusions:

The developed biomimetic EVMP nanoplatform offers a transformative advance in mRNA delivery technology for extrahepatic diseases.
This platform enables effective and safe mRNA-based therapies with programmable tissue tropism and modular functionality.
Establishes a generalizable strategy for engineering biomimetic materials for targeted therapeutic applications.