Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Osteoclasts in Bone Remodeling01:31

Osteoclasts in Bone Remodeling

4.9K
Osteoclasts are cells responsible for bone resorption and remodeling. They originate from hematopoietic progenitor cells present in the bone marrow. Numerous progenitor cells fuse to form multinucleated cells, each with 10-20 nuclei. A single osteoclast has a diameter of 150 to 200 µM. These cells have ruffled borders that break down the underlying bone tissue and release minerals such as calcium into the blood in bone resorption. Osteoclasts cling to bones with their ruffled edges during...
4.9K
Chronic Pancreatitis I: Introduction01:24

Chronic Pancreatitis I: Introduction

926
The pancreas, an elongated and flat gland situated behind the stomach, serves a vital function in digesting food and managing blood sugar levels.
Pancreatitis is the inflammation of the pancreas, which occurs when the immune system becomes active and causes swelling, pain, and disruptions in organ function. Pancreatitis can manifest as either an acute or chronic condition.
Acute pancreatitis arises suddenly and lasts for a brief duration, while chronic pancreatitis is a long-term affliction...
926
Bone Remodeling01:40

Bone Remodeling

41.3K
Bone remodeling is a continuous and balanced process of bone resorption by osteoclasts and bone formation by osteoblasts. In adults, it helps maintain bone mass and calcium homeostasis. While mechanical stress can stimulate turnover as part of the normal maintenance and reparative process, several hormones also regulate bone remodeling.
41.3K
Bone Cells and Tissue01:30

Bone Cells and Tissue

13.7K
Bones contain a relatively small number of cells entrenched in a matrix of organic and inorganic components. Although bone cells compose only a small amount of the bone volume, they are crucial to its function. Four types of cells are found within the bone tissue— osteoblasts, osteocytes, osteogenic cells, and osteoclasts.
Osteoblasts and Osteocytes
The osteoblast is the bone cell responsible for forming new bone tissue. It is found in the growing portions of bone, including the...
13.7K
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

17.3K
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
17.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Characterizing immune perturbations in peripheral blood following the East Palestine, Ohio train derailment.

Journal of exposure science & environmental epidemiology·2026
Same author

Spontaneous Pregravid Obesity Reshapes Fetal Immune Ontogeny in a Nonhuman Primate Model.

bioRxiv : the preprint server for biology·2026
Same author

Transcriptional signatures of rapid protection from Sudan virus infection by a single dose of a vesicular stomatitis virus-based vaccine.

PLoS pathogens·2026
Same author

Single-Cell Atlas of Fetal Immune Development Across Lung, Spleen, and Umbilical Cord Blood in Nonhuman Primates.

Research square·2026
Same author

Maternal Obesity Reprograms Differentiation Trajectories of Fetal Hematopoietic Stem and Progenitor Cells Through Altered Inflammatory Signaling.

bioRxiv : the preprint server for biology·2026
Same author

Short-Term Alcohol Consumption Is Sufficient to Disrupt Microbial Communities and the Intestinal and Circulating Metabolites in a Non-Human Primate Model.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2025

Related Experiment Video

Updated: Apr 14, 2026

Differentiation of Functional Osteoclasts from Human Peripheral Blood CD14+ Monocytes
11:52

Differentiation of Functional Osteoclasts from Human Peripheral Blood CD14+ Monocytes

Published on: January 27, 2023

4.9K

Chronic Alcohol Consumption Reprograms Osteoclast Lineage Communications to Promote Osteoclastogenesis.

Hami Hemati1, Brianna M Doratt1, Ilhem Messaoudi1

  • 1Microbiology, Immunology and Molecular Genetics, College of Medicine, University of Kentucky, Lexington, KY 40536, USA.

Biology
|April 13, 2026
PubMed
Summary

Chronic alcohol use enhances bone-resorbing osteoclast formation by altering their metabolism and cell communication. This study reveals how alcohol impacts osteoclast development, contributing to osteoporosis risk.

Keywords:
CellChatchronic alcohol consumptionnon-human primatesosteoclastosteoclast precursorsosteoclastogenesis

More Related Videos

Osteoclast Derivation from Mouse Bone Marrow
06:17

Osteoclast Derivation from Mouse Bone Marrow

Published on: November 6, 2014

25.2K
A Simple Pit Assay Protocol to Visualize and Quantify Osteoclastic Resorption In Vitro
07:03

A Simple Pit Assay Protocol to Visualize and Quantify Osteoclastic Resorption In Vitro

Published on: June 16, 2022

7.7K

Related Experiment Videos

Last Updated: Apr 14, 2026

Differentiation of Functional Osteoclasts from Human Peripheral Blood CD14+ Monocytes
11:52

Differentiation of Functional Osteoclasts from Human Peripheral Blood CD14+ Monocytes

Published on: January 27, 2023

4.9K
Osteoclast Derivation from Mouse Bone Marrow
06:17

Osteoclast Derivation from Mouse Bone Marrow

Published on: November 6, 2014

25.2K
A Simple Pit Assay Protocol to Visualize and Quantify Osteoclastic Resorption In Vitro
07:03

A Simple Pit Assay Protocol to Visualize and Quantify Osteoclastic Resorption In Vitro

Published on: June 16, 2022

7.7K

Area of Science:

  • Bone Biology
  • Cellular Metabolism
  • Alcohol-Induced Pathology

Background:

  • Chronic alcohol consumption is linked to increased osteoporosis and fracture risk.
  • Alcohol's detrimental effects on bone are partly due to enhanced osteoclastogenesis, but cellular mechanisms are unclear.

Purpose of the Study:

  • To investigate the cellular and molecular mechanisms by which chronic ethanol consumption alters osteoclast differentiation and function.
  • To identify ethanol-induced changes in metabolic reprogramming, lineage progression, and intercellular communication networks in osteoclasts.

Main Methods:

  • Analysis of single-cell RNA sequencing (scRNA-seq) data from osteoclasts differentiated in vitro from macaques exposed to chronic ethanol or control solutions.
  • Application of module scoring, trajectory inference (tradeSeq), and intercellular communication analysis (CellChat) to scRNA-seq data.

Main Results:

  • Ethanol consumption induced metabolic reprogramming in osteoclasts, favoring oxidative phosphorylation over glycolysis.
  • Osteoclast differentiation showed accelerated lineage commitment, broader intermediate states, and stabilization of mature cells.
  • Intercellular communication networks were globally amplified, with mature osteoclasts acting as central communication hubs via autocrine signaling.

Conclusions:

  • Chronic alcohol consumption reshapes osteoclastogenesis through metabolic adaptation and altered intercellular communication, promoting enhanced osteoclast activity.
  • These findings provide mechanistic insights into alcohol-induced bone disease and suggest potential therapeutic targets for mitigating bone loss.