Running an electron microscopy core facility

As electron microscopes became more costly, technically complex, and integral to a wide range of scientific fields, centralised electron microscopy (EM) core facilities have become essential for maintaining accessibility, performance, and quality. Drawing on the experiences of running two EM core facilities in Finland, we outline key considerations for establishing and operating a biological and biomedical EM facility. We cover facility infrastructure and technical requirements, user training, sample preparation, safety and occupational considerations, personnel and cost management, and finish by outlining three areas, sustainability, research data management, and artificial intelligence, that we consider to be increasingly defining the strategic direction of EM facilities. By distinguishing the specific requirements of EM from those of light microscopy, this article provides practical guidance to support efficient, safe, and high-quality facility operations within institutional and national imaging networks.