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  3. Overriding Bioprocess Perturbations With A Cell-machine Interface For Reliable Microbial Stress-response Control.
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  3. Overriding Bioprocess Perturbations With A Cell-machine Interface For Reliable Microbial Stress-response Control.

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Overriding Bioprocess Perturbations With a Cell-Machine Interface for Reliable Microbial Stress-Response Control.

Mathéo Delvenne1, Juan Andres Martinez1, Cees Haringa2

  • 1Terra Research and Teaching Centre, Microbial Processes and Interactions (MiPI), Gembloux Agro-Bio Tech, University of Liège, Gembloux, Belgium.

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|April 13, 2026

View abstract on PubMed

Summary
This summary is machine-generated.

This study demonstrates the Segregostat system

Keywords:
E. coliS. cerevisiaeSegregostatautomated flow cytometrybet‐hedgingcell lifelinelarge‐scale bioreactormicrobial stressscale‐downsingle cell

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Area of Science:

  • Systems and Synthetic Biology
  • Biotechnology
  • Microbial Engineering

Background:

  • Controlling cell population dynamics and phenotypic diversification is crucial for engineered gene circuits.
  • Industrial bioreactor conditions often involve environmental perturbations that impact cell behavior.
  • Existing cell-machine interfaces underexplored in the context of industrial bioreactor perturbations.

Purpose of the Study:

  • Investigate the impact of industrial bioreactor perturbations on the general stress response in Escherichia coli and Saccharomyces cerevisiae.
  • Evaluate the performance of the Segregostat real-time control system under these conditions.

Main Methods:

  • Scale-down bioreactor experiments.
  • Utilized the Segregostat system with automated flow cytometry for dynamic nutrient shifts.
  • Assessed stress response control under severe perturbations like extended residence times.

    • Main Results:

    • The Segregostat system demonstrated robust control of stress response.
    • Effective control was maintained even under severe bioreactor perturbations.
    • Hypothesized robustness stems from amplifying host-compatible fluctuations.

    Conclusions:

    • Environmental factors must be integrated into control strategies for reliable gene circuit behavior.
    • The Segregostat system shows promise for industrial bioprocessing.
    • Ensuring uniform responses from engineered gene circuits in industrial settings is achievable.