Identification of Modified Histones as Binding Substrates of Human Spindlin Family Member 4 (SPIN4) by Peptide Arrays...

Human Spindlin Family Member 4 (SPIN4) has just emerged as a novel regulator of bone growth. Loss-of-function variants in SPIN4 were identified in patients exhibiting a pre- and post-natal overgrowth syndrome, which is inherited in an X-linked dominant fashion. SPIN4 contains three tandem repeats of Tudor domains, each forming an individual aromatic cage. These Tudor domains are considered epigenetic reader domains for methylated arginine and lysine residues of histones based on sequence homology. However, the exact binding substrates of SPIN4 remain unclear. Human SPIN4, in both its wild-type and disease-causing mutant forms, was successfully generated via an insect cell expression system. By a histone modification peptide array, the differential binding affinities between wild-type and mutant SPIN4 were determined. Functional validations further demonstrated that SPIN4 is a bona fide reader of histone H3 Lysine 4 methylation (H3K4me), as evidenced by biochemical pulldown of native mononucleosomes. This integrated approach facilitates a rapid binding target identification of epigenetic reader proteins.